Home

Citation: Yifan Xie, Liyun Yao, Zhonghong Yan, Yuxing Cai, Yujie Jin, Ning Li. 超高效合相色谱法测定咖啡因含量实验设计[J]. University Chemistry, ;2021, 36(9): 201105. doi: 10.3866/PKU.DXHX202011055 shu

超高效合相色谱法测定咖啡因含量实验设计

Yifan Xie ¹ ,
Liyun Yao ^1,2,, ,
Zhonghong Yan ² ,
Yuxing Cai ¹ ,
Yujie Jin ¹ ,
Ning Li ¹

1.
Basic Medical Experimental Teaching Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China;
2.
Medicinal Chemistry and Bioinformatics Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China

Corresponding author: Liyun Yao, yaoliyun517@163.com
Received Date: 25 November 2020

设计了一个仪器分析实验，利用超高效合相色谱仪（Ultra Performance Convergence Chromatography，UPCC或UPC²）测定饮料中咖啡因的含量。该实验选取可乐、绿茶、乌龙茶三种饮料，样品用甲醇萃取，萃取液无需旋蒸，直接稀释定容后进样，测定其中咖啡因的含量。通过该实验学生不仅能巩固称量、定容、移液等基本操作，还能掌握UPC²的基本原理、操作及应用色谱软件处理数据的技能。本实验将新型色谱仪UPC²应用于基础实验教学，可以拓展学生的科学视野，有利于培养学生的创新精神和实践能力。
- UPC²,
- Caffeine,
- Content determination,
- Laboratory teaching
1. [1]
  Joseph, G. L.; Grace, E. L.; Clifford, H. W. Nicotine Tob. Res. 2017, 4(19), 484.
2. [2]
  Verenitch, S. S.; Mazumder, A. Sleep 2017, 40(1), 1.
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
1. [1]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Application of Comparative Pedagogy in Instrumental Analysis Experiment Teaching. University Chemistry, 2024, 39(3): 266-273. doi: 10.3866/PKU.DXHX202309068
2. [2]
  Zhongbin Pan , Shijie Huang , Yunjie Luo , Hongzhen Xie . Design of a Comprehensive Experiment for Determining Permanganate Index (COD_Mn) in Drinking Water. University Chemistry, 2024, 39(7): 354-360. doi: 10.12461/PKU.DXHX202311040
3. [3]
  Haorui Gu , Ning Li , Zhanxiang Liu , Xufeng Lin . Construction and Development of Chemistry Experimental Teaching Center under the Background of the “101 Plan”. University Chemistry, 2024, 39(7): 110-115. doi: 10.12461/PKU.DXHX202405022
4. [4]
  Yan Zhao , Weiping Luo , Haoran Liu , Yongqing Kuang , Zhaoyang Wu , Weijun Yang , Yongjun Li , Dongcai Guo . Construction and Practice of the Chemistry and Chemical Engineering Experimental Teaching Center of Hunan University. University Chemistry, 2024, 39(7): 147-152. doi: 10.12461/PKU.DXHX202405059
5. [5]
  Dongcheng Liu , Xiaokun Li , Huancheng Hu , Cunji Gao , Qiong Hu , Shuting Li , Yuning Liang . Chemistry Experimental Teaching Reform for the Promotion of Training Exceptional Chemistry Teachers for Normal Schools. University Chemistry, 2024, 39(8): 1-6. doi: 10.3866/PKU.DXHX202311072
6. [6]
  Bin Wang , Chuanli Qin , Naiying Fan , Zhibin Li , Zhibiao Zhu , Jiancong Liu , Shaoping Sun . Construction and Practice of Digital Platform and Teaching Resources of Chemistry Experimental Teaching Center: A Case Study of Provincial Demonstration Center for Experimental Chemistry Education (Heilongjiang University). University Chemistry, 2024, 39(7): 193-199. doi: 10.12461/PKU.DXHX202405116
7. [7]
  Zunyuan Xie , Lijin Yang , Zixiao Wan , Xiaoyu Liu , Yushan He . Exploration of the Preparation and Characterization of Nano Barium Titanate and Its Application in Inorganic Chemistry Laboratory Teaching. University Chemistry, 2024, 39(4): 62-69. doi: 10.3866/PKU.DXHX202310137
8. [8]
  Wei Zhang , Kunying Xing , Wei Li , Yan Geng , Bo Tang . Construction and Practice of National Experimental Teaching Demonstration Center of Chemistry (Shandong Normal University). University Chemistry, 2024, 39(7): 20-25. doi: 10.12461/PKU.DXHX202404059
9. [9]
  Heng Zhang , Gang Liu , Zhenghu Xu , Ying Ma . Construction and Practice of Comprehensive Open and Innovative Chemistry Experimental Teaching System and Platform in the Context of Multidisciplinary Integration. University Chemistry, 2024, 39(7): 56-63. doi: 10.12461/PKU.DXHX202405001
10. [10]
  Xiaohui Li , Ze Zhang , Jingyi Cui , Juanjuan Yin . Advanced Exploration and Practice of Teaching in the Experimental Course of Chemical Engineering Thermodynamics under the “High Order, Innovative, and Challenging” Framework. University Chemistry, 2024, 39(7): 368-376. doi: 10.3866/PKU.DXHX202311027
11. [11]
  Zhangshu Wang , Xin Zhang , Jixin Han , Xuebing Fang , Xiufeng Zhao , Zeyu Gu , Jinjun Deng . Exploration and Design of Experimental Teaching on Ultrasonic-Enhanced Synergistic Treatment of Ternary Composite Flooding Produced Water. University Chemistry, 2024, 39(5): 116-124. doi: 10.3866/PKU.DXHX202310056
12. [12]
  Tong Zhou , Liyi Xie , Chuyu Liu , Xiyan Zheng , Bao Li . Between Sobriety and Intoxication: The Fascinating Journey of Sauce-Flavored Latte. University Chemistry, 2024, 39(9): 55-58. doi: 10.12461/PKU.DXHX202312048
13. [13]
  Zhenlin Zhou , Siyuan Chen , Yi Liu , Chengguo Hu , Faqiong Zhao . A New Program of Voltammetry Experiment Teaching Based on Laser-Scribed Graphene Electrode. University Chemistry, 2024, 39(2): 358-370. doi: 10.3866/PKU.DXHX202308049
14. [14]
  Dongxue Han , Zhuoyong Li , Hanbo Zou , Xu Wu , Yang Yuan , Hongbin Li . Research on Innovative Experimental Teaching to Cultivate Top Talents. University Chemistry, 2024, 39(7): 230-236. doi: 10.12461/PKU.DXHX202406094
15. [15]
  Laiying Zhang , Yinghuan Wu , Yazi Yu , Yecheng Xu , Haojie Zhang , Weitai Wu . Innovation and Practice of Polymer Chemistry Experiment Teaching for Non-Polymer Major Students of Chemistry: Taking the Synthesis, Solution Property, Optical Performance and Application of Thermo-Sensitive Polymers as an Example. University Chemistry, 2024, 39(4): 213-220. doi: 10.3866/PKU.DXHX202310126
16. [16]
  Yeyun Zhang , Ling Fan , Yanmei Wang , Zhenfeng Shang . Development and Application of Kinetic Reaction Flasks in Physical Chemistry Experimental Teaching. University Chemistry, 2024, 39(4): 100-106. doi: 10.3866/PKU.DXHX202308044
17. [17]
  Dexin Tan , Limin Liang , Baoyi Lv , Huiwen Guan , Haicheng Chen , Yanli Wang . Exploring Reverse Teaching Practices in Physical Chemistry Experiment Courses: A Case Study on Chemical Reaction Kinetics. University Chemistry, 2024, 39(11): 79-86. doi: 10.12461/PKU.DXHX202403048
18. [18]
  Wenjun Yang , Qiaoling Tan , Wenjiao Xie , Xiaoyu Pan , Youyong Yuan . Construction and Characterization of Calcium Alginate Microparticle Drug Delivery System: A Novel Design and Teaching Practice in Polymer Experiments. University Chemistry, 2025, 40(3): 371-380. doi: 10.12461/PKU.DXHX202405150
19. [19]
  Zhonghua Xi , Xuanfeng Kong , Jinyue Yang , Bin Liu , Tingyu Zhu , Hui Zhang , Wenwei Zhang . Construction of Public Teaching Instrument Platform and Exploration of Opening Mechanism. University Chemistry, 2024, 39(7): 200-206. doi: 10.12461/PKU.DXHX202405123
20. [20]
  Zhihui Wen , Zhanheng Feng , Xue Qi , Xiaohang Qiu . Exploration and Practice in Inorganic Chemistry Laboratory Management under Broad-based Admission Programs. University Chemistry, 2024, 39(6): 181-188. doi: 10.3866/PKU.DXHX202310081

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(994)
HTML views(142)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return