Citation: Guosheng Song, Shiyi Liao, Youjuan Wang, Shuangyan Huan. 基于半导体聚合物纳米粒子探针的比率型活性物种检测——推荐一个研究型综合化学实验[J]. University Chemistry, ;2021, 36(8): 201103. doi: 10.3866/PKU.DXHX202011032 shu

基于半导体聚合物纳米粒子探针的比率型活性物种检测——推荐一个研究型综合化学实验

College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

Received Date: 9 November 2020

介绍一个从纳米探针的合成、表征、性质测试到应用研究的完整研究型综合实验。本实验利用纳米共沉淀法制备了有机聚合物纳米探针，用有机半导体聚合物（PFODBT）作为参比分子和荧光染料分子（IR775s）作为响应分子，构建吸光度和荧光双比率型探针，实现了对活性氧物种（ClO^-和ONOO^-）含量的检测。采用紫外-可见吸收光谱仪可实现比率型吸光度检测活性氧物种的含量。利用PFODBT和IR775s之间的荧光共振能量转移（FRET）原理，采用荧光光谱仪可实现比率型荧光检测活性氧物种的含量。该实验综合性强、操作简便、可行性好、有利于培养学生全面的实验技能和科研能力。
- Analytical measurement,
- Nanoprobe,
- Fluorescence resonance energy transfer,
- Ratiometric detection,
- Reactive oxygen species
1. [1]
  Jiang, Y.; Cui, D.; Fang, Y.; Zhen, X.; Upputuri, P. K.; Pramanik, M.; Ding, D.; Pu, K.Biomaterials 2017, 145, 168.
2. [2]
  Shuhendler, A. J.; Pu, K.; Cui, L.; Uetrecht, J. P.; Rao, J.Nat. Biotechnol.2014, 32, 373.
3. [3]
  Lyu, Y.; Cui, D.; Sun, H.; Miao, Y.; Duan, H.; Pu, K.Angew. Chem. Int. Ed.2017, 56,9155.
4. [4]
  Pu, K.; Shuhendler, A. J.; Jokerst, J. V.; Mei, J.; Gambhir, S. S.; Bao, Z.; Rao, J.Nat. Nanotechnol.2014, 92, 33.
5. [5]
  Wang, J.; Yi, J.Cancer Biol. Ther.2008, 7, 1875.
6. [6]
  Setsukinai, K.; Urano, Y.; Kakinuma, K.; Majima, H. J.; Nagano, T.J. Biol. Chem.2003, 278, 3170.
7. [7]
  Wang, Y.; Shi, L.; Ye, Z.; Guan, K.; Teng, L.; Wu, J.; Yin, X.; Song, G.; Zhang, X. B.Nano Lett.2020, 20, 176.
8. [8]
  Kubo, N.; Morita, M.; Nakashima, Y.; Kitao, H.; Egashira, A.; Saeki, H.; Oki, E.; Kakeji, Y.; Oda, Y.; Maehara, Y.Dis. Esophagus 2014, 27, 285.
9. [9]
  Nakabeppu, Y.; Tsuchimoto, D.; Ichinoe, A.; Ohno, M.; Ide, Y.; Hirano, S.; Yoshimura, D.; Tominaga, Y.; Furuichi, M.; Sakumi, K.Ann. N. Y. Acad.sci.2004, 1011, 101.
10. [10]
  Houstis, N.; Rosen, E. D.; Lander, E. S.Nature 2006, 440, 944.
11. [11]
  Pu, K.; Shuhendler, A. J.; Rao, J.Angew. Chem. Int. Ed.2013, 52, 10325.
12. [12]
  Oushiki, D.; Kojima, H.; Terai, T.; Arita, M.; Hanaoka, K.; Urano, Y.; Nagano, T.J. Am. Chem. Soc.2010, 1322, 795.
13. [13]
  Dagher, M.; Kleinman, M.; Ng, A.; Juncker, D.Nat. Nanotechnol.2018, 13, 925.
14. [14]
  Cheng, D.; Pan, Y.; Wang, L.; Zeng, Z.; Yuan, L.; Zhang, X.; Chang, Y.-T.J. Am. Chem. Soc.2017, 139, 285.
1. [1]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249
2. [2]
  Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
3. [3]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
4. [4]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
5. [5]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
6. [6]
  Kuaibing Wang , Honglin Zhang , Wenjie Lu , Weihua Zhang . Experimental Design and Practice for Recycling and Nickel Content Detection from Waste Nickel-Metal Hydride Batteries. University Chemistry, 2024, 39(11): 335-341. doi: 10.12461/PKU.DXHX202403084
7. [7]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
8. [8]
  Weihan Zhang , Menglu Wang , Ankang Jia , Wei Deng , Shuxing Bai . 表面硫物种对钯-硫纳米片加氢性能的影响. Acta Physico-Chimica Sinica, 2024, 40(11): 2309043-. doi: 10.3866/PKU.WHXB202309043
9. [9]
  Qin Hou , Jiayi Hou , Aiju Shi , Xingliang Xu , Yuanhong Zhang , Yijing Li , Juying Hou , Yanfang Wang . Preparation of Cuprous Iodide Coordination Polymer and Fluorescent Detection of Nitrite: A Comprehensive Chemical Design Experiment. University Chemistry, 2024, 39(8): 221-229. doi: 10.3866/PKU.DXHX202312056
10. [10]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
11. [11]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
12. [12]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
13. [13]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
14. [14]
  Tongyu Zheng , Teng Li , Xiaoyu Han , Yupei Chai , Kexin Zhao , Quan Liu , Xiaohui Ji . A DIY pH Detection Agent Using Persimmon Extract for Acid-Base Discoloration Popularization Experiment. University Chemistry, 2024, 39(5): 27-36. doi: 10.3866/PKU.DXHX202309107
15. [15]
  Qin Tu , Anju Tao , Tongtong Ma , Jinyi Wang . Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip. University Chemistry, 2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062
16. [16]
  Zhening Lou , Quanxing Mao , Xiaogeng Feng , Lei Zhang , Xu Xu , Yuyang Zhang , Xueyan Liu , Hongling Kang , Dongyang Feng , Yongku Li . Practice of Implementing Blended Teaching in Shared Analytical Chemistry Course. University Chemistry, 2024, 39(2): 263-269. doi: 10.3866/PKU.DXHX202308089
17. [17]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
18. [18]
  Hao BAI , Weizhi JI , Jinyan CHEN , Hongji LI , Mingji LI . Preparation of Cu₂O/Cu-vertical graphene microelectrode and detection of uric acid/electroencephalogram. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1309-1319. doi: 10.11862/CJIC.20240001
19. [19]
  Mengyao Shi , Kangle Su , Qingming Lu , Bin Zhang , Xiaowen Xu . Determination of Potassium Content in Tobacco Stem Ash by Flame Atomic Absorption Spectroscopy. University Chemistry, 2024, 39(10): 255-260. doi: 10.12461/PKU.DXHX202404105
20. [20]
  Li'na ZHONG , Jingling CHEN , Qinghua ZHAO . Synthesis of multi-responsive carbon quantum dots from green carbon sources for detection of iron ions and L-ascorbic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 709-718. doi: 10.11862/CJIC.20240280

Get Citation

PDF

XML

Metrics

PDF Downloads(5)
Abstract views(1001)
HTML views(81)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142