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Citation: LIU Yang. Characterization of DNA Polymerases Activities by DNA Genetic Analyzer[J]. University Chemistry, ;2017, 32(8): 42-48. doi: 10.3866/PKU.DXHX201701021 shu

Characterization of DNA Polymerases Activities by DNA Genetic Analyzer

LIU Yang

Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China

In this paper, one comprehensive experiment was proposed for teaching the chemical biology laboratory. By utilizing 6-FAM labelled primer and DNA genetic analyzer, we studied the effects of DNA polymerases structure on the replication of damaged DNA. The application of this experiment to laboratory teaching can cultivate students' comprehensive analysis and hands-on abilities.
- Benzopyrene,
- Translesion DNA synthesis,
- Teaching experiment
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