Citation: SUN Yun-Xia, ZHANG Xian-Zheng. Synthesis and Biological Research of Polycationic Oligopeptide: A Research-Based Experiment Recommended for Chemical Biology Majoring Students[J]. University Chemistry, ;2016, 31(3): 42-48. doi: 10.3866/PKU.DXHX20160342 shu

Synthesis and Biological Research of Polycationic Oligopeptide: A Research-Based Experiment Recommended for Chemical Biology Majoring Students

SUN Yun-Xia ^, ,
ZHANG Xian-Zheng

1.
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China

Corresponding author: SUN Yun-Xia,
Fund Project: 国家自然科学基金项目(21004046,51473126) (21004046,51473126)

The oligopeptide R₈ was synthesized through the solid phase peptide synthesis (SPPS) protocols, and the molecular weight was measured by MS-ESI. The biological application of R₈ was investigated by agarose gel electrophoresis assay and endocytosis. Through this experiment, students can study and apply organic chemistry, polymer chemistry, and chemical biology, as well as develop their capability for analyzing and resolving problems.
- Amino acid,
- Oligopeptide,
- Peptide,
- Gel electrophoresis
1. [1]
  [1] 李世成, 杨则宜. 中国运动医学杂志, 2003, No. 2, 174.
2. [2]
  [2] Ganapathy, V.; Leibach, F. H. J. Biol. Chem. 1983, 258(23), 14189.
3. [3]
  [3] Mahato, R. I. Adv. Drug. Deliv. Rev. 2005, 57, 699.
4. [4]
  [4] Mintzer, M. A.; Simanek, E. E. Chem. Rev. 2009, 109, 259.
5. [5]
  [5] Du, L. B.; Gao, Y. L.; Yang, H.; Li, Y. C.; Zhuang, Q. F.; Jia, H. Y.; Nie, G. J.; Liu, Y. RSC Adv. 2013, 3(34), 14791.
6. [6]
  [6] Layek, B.; Singh, J. Biomacromolecules 2013, 14, 485.
7. [7]
  [7] Zhou, D. Z.; Li, C. X.; Hu, Y. L.; Zhou, H.; Chen, J. T.; Zhang, Z. P.; Guo, T. Y. Chem. Commun. 2012, 48, 4594.
8. [8]
  [8] Cai, X. J.; Li, Y. Y.; Yue, D.; Yi, Q. Y.; Li, S.; Shi, D. L.; Gu, Z.W. J. Mater. Chem. B 2015, 3(8), 1507.
9. [9]
  [9] Yamada, H.; Loretz, B.; Lehr, C. M. Biomacromolecules 2014, 15(5), 1753.
10. [10]
  [10] Bao, X. L.;Wang,W.;Wang, C.;Wang, Y.; Zhou, J. P.; Ding, Y.;Wang, X. Y.; Jin, Y. T. Biomaterials 2014, 35(29), 8450.
11. [11]
  [11] Lee, J.; Jung, J.; Kim, Y. J.; Lee, E.; Choi, J. S. Int. J. Pharmaceut. 2014, 459(1-2), 10.
12. [12]
  [12] Kircheis, R.;Wightman, L.;Wagner, E. Adv. Drug Deliv. Rev. 2001, 53, 341.
13. [13]
  [13] 王昱佳, 刘欣宇, 陈永湘. 大学化学, 2014, 29(2), 54.
14. [14]
  [14] Lehto, T.; Simonson, O. E.; Mäger, I.; Ezzat, K.; Sork, H.; Copolovici, D. M.; Viola, J. R.; Zaghloul, E. M.; Lundin, P.; Moreno, P. M. D.; Mäe, M.; Oskolkov, N.; Suhorutšenko, J.; Smith, C. I.; Andaloussi, S. E. L. Mol. Ther. 2011, 19(8), 1457.
15. [15]
  [15] Tiwari, P. M.; Eroglu, E.; Bawage, S. S. Biomaterials 2014, 35, 9484.
16. [16]
  [16] Fields, G. B.; Noble, R. L. Int. J. Pept. Protein Res. 1990, 35(3), 161.
17. [17]
  [17] Moore, N. M.; Barbour, T. R.; Sakiyama-Elbert, S. E. Mol. Pharm. 2008, 5(1), 140.
18. [18]
  [18] Park, T. G.; Jeong, J. H.; Kim, S.W. Adv. Drug Deliv. Rev. 2006, 58, 467.
1. [1]
  Hong CAI , Jiewen WU , Jingyun LI , Lixian CHEN , Siqi XIAO , Dan LI . Synthesis of a zinc-cobalt bimetallic adenine metal-organic framework for the recognition of sulfur-containing amino acids. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 114-122. doi: 10.11862/CJIC.20240382
2. [2]
  Zihan Lin , Wanzhen Lin , Fa-Jie Chen . Electrochemical Modifications of Native Peptides. University Chemistry, 2025, 40(3): 318-327. doi: 10.12461/PKU.DXHX202406089
3. [3]
  Wenjun Yang , Qiaoling Tan , Wenjiao Xie , Xiaoyu Pan , Youyong Yuan . Construction and Characterization of Calcium Alginate Microparticle Drug Delivery System: A Novel Design and Teaching Practice in Polymer Experiments. University Chemistry, 2025, 40(3): 371-380. doi: 10.12461/PKU.DXHX202405150
4. [4]
  Yuena Yang , Xufang Hu , Yushan Liu , Yaya Kuang , Jian Ling , Qiue Cao , Chuanhua Zhou . The Realm of Smart Hydrogels. University Chemistry, 2024, 39(5): 172-183. doi: 10.3866/PKU.DXHX202310125
5. [5]
  Yunchao Li , Shanying Chen , Ke Qi , Kangning Huo , Shuxin Li , Jingyi Li , Ying Wei , Louzhen Fan . A New Colloid Electrophoresis Experiment Incorporating Characteristics of Inquiry Learning and Ideological and Political Education. University Chemistry, 2024, 39(2): 47-51. doi: 10.3866/PKU.DXHX202308063
6. [6]
  Shanying Chen , Kangning Huo , Ke Qi , Jingyi Li , Shuxin Li , Yunchao Li . A Novel Colloid Electrophoresis Experiment with the Characteristics of Resource Recycling and Inquiry-Driven Experimental Design. University Chemistry, 2024, 39(5): 274-286. doi: 10.3866/PKU.DXHX202311067
7. [7]
  Feng Liang , Desheng Li , Yuting Jiang , Jiaxin Dong , Dongcheng Liu , Xingcan Shen . Method Exploration and Instrument Innovation for the Experiment of Colloid ζ Potential Measurement by Electrophoresis. University Chemistry, 2024, 39(5): 345-353. doi: 10.3866/PKU.DXHX202312009
8. [8]
  Qingying Gao , Tao Luo , Jianyuan Su , Chaofan Yu , Jiazhu Li , Bingfei Yan , Wenzuo Li , Zhen Zhang , Yi Liu . Refinement and Expansion of the Classic Cinnamic Acid Synthesis Experiment. University Chemistry, 2024, 39(5): 243-250. doi: 10.3866/PKU.DXHX202311074
9. [9]
  Yongqing Kuang , Jie Liu , Jianjun Feng , Wen Yang , Shuanglian Cai , Ling Shi . Experimental Design for the Two-Step Synthesis of Paracetamol from 4-Hydroxyacetophenone. University Chemistry, 2024, 39(8): 331-337. doi: 10.12461/PKU.DXHX202403012
10. [10]
  Dong-Bing Cheng , Junxin Duan , Haiyu Gao . Experimental Teaching Design on Chitosan Extraction and Preparation of Antibacterial Gel. University Chemistry, 2024, 39(2): 330-339. doi: 10.3866/PKU.DXHX202308053
11. [11]
  Tengjiao Wang , Tian Cheng , Rongjun Liu , Zeyi Wang , Yuxuan Qiao , An Wang , Peng Li . Conductive Hydrogel-based Flexible Electronic System: Innovative Experimental Design in Flexible Electronics. University Chemistry, 2024, 39(4): 286-295. doi: 10.3866/PKU.DXHX202309094
12. [12]
  Qiang Zhou , Pingping Zhu , Wei Shao , Wanqun Hu , Xuan Lei , Haiyang Yang . Innovative Experimental Teaching Design for 3D Printing High-Strength Hydrogel Experiments. University Chemistry, 2024, 39(6): 264-270. doi: 10.3866/PKU.DXHX202310064
13. [13]
  Qingyang Cui , Feng Yu , Zirun Wang , Bangkun Jin , Wanqun Hu , Wan Li . From Jelly to Soft Matter: Preparation and Properties-Exploring of Different Kinds of Hydrogels. University Chemistry, 2024, 39(9): 338-348. doi: 10.3866/PKU.DXHX202309046
14. [14]
  Haoxiang Zhang , Zhihan Zhao , Yongchen Jin , Zhiqiang Niu , Jinlei Tian . Synthesis of an Efficient Absorbent Gel: A Recommended Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(11): 251-258. doi: 10.12461/PKU.DXHX202401084
15. [15]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
16. [16]
  Keying Qu , Jie Li , Ziqiu Lai , Kai Chen . Unveiling the Mystery of Chirality from Tartaric Acid. University Chemistry, 2024, 39(9): 369-378. doi: 10.12461/PKU.DXHX202310091
17. [17]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . 杂多酸H₃PW₁₂O₄₀高效催化MgH₂储氢. Acta Physico-Chimica Sinica, 2025, 41(1): 2308032-. doi: 10.3866/PKU.WHXB202308032
18. [18]
  Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
19. [19]
  Jing SU , Bingrong LI , Yiyan BAI , Wenjuan JI , Haiying YANG , Zhefeng Fan . Highly sensitive electrochemical dopamine sensor based on a highly stable In-based metal-organic framework with amino-enriched pores. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1337-1346. doi: 10.11862/CJIC.20230414
20. [20]
  Guoqiang Chen , Zixuan Zheng , Wei Zhong , Guohong Wang , Xinhe Wu . 熔融中间体运输导向合成富氨基g-C₃N₄纳米片用于高效光催化产H₂O₂. Acta Physico-Chimica Sinica, 2024, 40(11): 2406021-. doi: 10.3866/PKU.WHXB202406021

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(418)
HTML views(42)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142