Citation: BAI Jie, WANG Da, LIU Zeping, ZHANG Jiaqi, LIU Liyan, HAN Yanmei. Simultaneous determination of amino acid and monoamine neurotransmitters in serum by high performance liquid chromatography coupled with precolumn derivatization[J]. Chinese Journal of Chromatography, ;2020, 38(8): 923-928. doi: 10.3724/SP.J.1123.2019.12029 shu

Simultaneous determination of amino acid and monoamine neurotransmitters in serum by high performance liquid chromatography coupled with precolumn derivatization

BAI Jie ^1,2,, ,
WANG Da ¹ ,
LIU Zeping ¹ ,
ZHANG Jiaqi ¹ ,
LIU Liyan ² ,
HAN Yanmei ^2,,

1.
College of Public Health, Hebei University, Baoding 071000, China;
2.
Medical Comprehensive Experimental Center, Hebei University, Baoding 071000, China

Corresponding author: BAI Jie, HAN Yanmei,
Received Date: 23 December 2019

Fund Project: National Natural Science Foundation of China (No. 21605035)

Using o-phthalaldehyde (OPA) as the derivatization reagent, a precolumn derivatized -high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of amino acid neurotransmitters taurine (Tau), glutamic acid (Glu), glycine(Gly), and γ -aminobutyric acid (γ-GABA), as well as the monoamine neurotransmitter dopamine (DA), in serum samples. The samples and ethanol were mixed at a volume ratio of 1:2 (v/v) for protein precipitation. After centrifugation, the supernatant was withdrawn and blown to dryness using nitrogen. The residue was pre-column derivatized with OPA, and the derivatized product was isolated by gradient elution ona Luna 5u C18 column (250 mm×4.6 mm, 5 μm). Under the optimal experimental conditions, the five neurotransmitters showed good linearities (r² ≥ 0.9866). The limits of detection were between 0.10 and 0.40 μmol/L. The spiked recoveries at different spiked levels were 87.57%-115.31%, and the RSDs were below 7.80%. This method is simple, sensitive, and it can be promised for the simultaneous detection of amino acid and monoamine neurotransmitters.
- high performance liquid chromatography (HPLC),
- precolumn derivatization,
- amino acid neurotransmitter,
- monoamine neurotransmitters,
- o-phthalaldehyde (OPA),
- serum
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