Citation: FANG Zhen, QU Li, GU Shuqing, CHEN Rouhan, LI You, DENG Xiaojun, GUO Dehua, FENG Feng. Determination of 16 mycotoxins in drug and food homologous products by ultra performance liquid chromatography-tandem mass spectrometry combined with accelerated solvent extraction and QuEChERS[J]. Chinese Journal of Chromatography, ;2020, 38(7): 782-790. doi: 10.3724/SP.J.1123.2019.10034 shu

Determination of 16 mycotoxins in drug and food homologous products by ultra performance liquid chromatography-tandem mass spectrometry combined with accelerated solvent extraction and QuEChERS

FANG Zhen ¹ ,
QU Li ¹ ,
GU Shuqing ^1,, ,
CHEN Rouhan ² ,
LI You ¹ ,
DENG Xiaojun ¹ ,
GUO Dehua ¹ ,
FENG Feng ³

1.
Technical Center for Animal Plant and Food Inspection and Quarantine, Shanghai Customs, Shanghai 200135, China;
2.
Department of Food Engineering, School of Life Science, Shanghai University, Shanghai 200436, China;
3.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China

Corresponding author: GU Shuqing, gushuqing@sina.cn
Received Date: 30 October 2019

Fund Project: National Key Research and Development Plan Project (No. 2017YFF0211003)

A method was developed for the simultaneous determination of 16 mycotoxins in drug and food homologous products by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with accelerated solvent extraction (ASE) and QuEChERS. The target mycotoxins in drug and food homologous products were extracted by ASE. After concentration, the extracts were purified by QuEChERS. Then, the target compounds were analyzed by UPLC-MS/MS in both positive and negative electrospray ionization and MRM modes. Aflatoxin B1 and fumonisin B1 were quantified by the internal standard method, and the remaining mycotoxins were quantified by the matrix-matched external standard method. The proposed method showed a good linear relationship, with correlation coefficients greater than 0.99. The limits of detection (LODs) and limits of quantification (LOQs) of the 16 mycotoxins ranged from 0.008 μg/kg to 0.3 μg/kg and from 0.03 μg/kg to 1.0 μg/kg, respectively. The blank samples were spiked at three levels, and the recoveries ranged from 70.8% to 118%, with the RSDs being 2.5% to 10.2%. The developed method was successfully applied to mycotoxin analysis in 30 scutellaria, puerarin and sea buckthorn samples bought from local markets. Different levels of mycotoxins were detected in some of the products. The proposed method is simple, rapid and sensitive, and it can be applied to the simultaneous determination of multi-mycotoxins in drug and food homologous products.
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