Citation: FU Hui, LU Yifu, HU Xiaojian, ZHANG Xu, YANG Yanwei, ZHU Ying. Determination of polycyclic aromatic hydrocarbon metabolites in urine by liquid-liquid extraction-high resolution gas chromatography-high resolution dual-focus magnetic mass spectrometry[J]. Chinese Journal of Chromatography, ;2020, 38(6): 715-721. doi: 10.3724/SP.J.1123.2019.10031 shu

Determination of polycyclic aromatic hydrocarbon metabolites in urine by liquid-liquid extraction-high resolution gas chromatography-high resolution dual-focus magnetic mass spectrometry

National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China

Corresponding author: ZHU Ying, zhuying@nieh.chinacdc.cn
Received Date: 29 October 2019

Fund Project: National Program for Central Financial Fund of China (No. 131031108000150003).

A sensitive and accurate method was developed to quantify eight polycyclic aromatic hydrocarbon (PAH) metabolites in human urine by liquid-liquid extraction-high resolution gas chromatography-high resolution dual-focus magnetic mass spectrometry (LLC-HRGC-HRMS). About 2 mL urine was mixed with a deuterium- or ¹³C-labeled isotopic internal standard, and the conjugated targets were enzymatically digested in the presence of ascorbic acid. The free compounds were extracted with toluene-pentane (1:4, v/v) and condensed to near dryness. Then, the target compounds were redissolved in toluene. After derivatization, they were separated and quantified by HRGC-HRMS. The linear ranges of the 1-hydroxynaphthalene, 1-hydroxyphenanthrene, and 2-hydroxyphenanthrene were 0.14-41.6 μg/L, 0.05-8.33 μg/L, and 0.04-8.33 μg/L, respectively, and those for the other five PAH metabolites were 0.02-8.33 μg/L. The correlation coefficients were greater than 0.99. The limits of detection were in the range of 0.006-0.042 μg/L, and the recoveries were 81.4%-127.0%. The intra-day and inter-day relative standard deviations (RSDs) were less than 6.9% and 10.9%, respectively. This method was utilized for the determination of 330 human urine samples. The results showed that 3-hydroxychrysene and 6-hydroxychrysene were not detected, and the detection rate of the other six PAH metabolites was 100%. This method is sensitive, accurate and stable, and it is suitable for the determination of the eight PAH metabolites in human urine.
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
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