Citation: ZHAO Nan, CHENG Mengchun, WU Yulin, LIU Dan, ZHANG Xiaozhe. Differential analysis of peptides in Panax ginseng C. A. Meyer root by ultra-performance liquid chromatography-high resolution mass spectrometry[J]. Chinese Journal of Chromatography, ;2019, 37(12): 1305-1313. doi: 10.3724/SP.J.1123.2019.09006 shu

Differential analysis of peptides in Panax ginseng C. A. Meyer root by ultra-performance liquid chromatography-high resolution mass spectrometry

ZHAO Nan ^1,2, ,
CHENG Mengchun ¹ ,
WU Yulin ^1,3 ,
LIU Dan ¹ ,
ZHANG Xiaozhe ¹

1.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
Henan University of Chinese Medicine, Zhengzhou 450046, China

Received Date: 6 September 2019

Fund Project: National Natural Science Foundation of China (Nos. 81573830, 81774154).

An ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS)-based peptidomics approach was developed for evaluating the similarities and differences between peptides in different morphological regions of Panax ginseng C. A. Meyer root. A total of 62 high-confidence peptides were characterized through database search, and four parts of ginseng root were all rich in peptides. The results of the differential analysis showed that the peptides were differentially expressed between the main root (MR), branch root (BR), rhizome (RH), and fibrous root (FR). Besides, a total of 25 potential peptide markers enabling the differentiation were discovered. In particular, the difference between the MR and the other parts was the most significant, and it led to a new viewpoint for the study of the difference in pharmacodynamics between the MR and the non-MR. These findings illustrate the structural diversity of ginseng peptides and reveal the similarities and differences between the peptides in ginseng root, thus providing chemical evidence for the quality control and rational application of ginseng.
- ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS),
- peptides,
- Panax ginseng,
- peptidomics
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