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Citation: WANG Xiyue, MING Ming, LIAN Lili, ZHANG Hao, LOU Dawei. Analysis of fatty acids in rice by pseudotargeted metabolomics method based on gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2020, 38(2): 250-254. doi: 10.3724/SP.J.1123.2019.05041 shu

Analysis of fatty acids in rice by pseudotargeted metabolomics method based on gas chromatography-mass spectrometry

College of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China

Corresponding author: LOU Dawei, dwlou@hotmail.com
Received Date: 26 May 2019

Fund Project: National Natural Science Foundation of China (Nos. 21375046, 21605056)

A pseudotargeted metabolomics method based on gas chromatography-mass spectrometry (GC-MS) was developed for the analysis of fatty acids in rice. A total of 16 fatty acids were detected in rice. The method was used to identify the difference between fatty acid profiles in rice. The total intensity of the saturated and unsaturated fatty acids was used as the evaluation index. The effects of six extraction methods and four extraction solvents on the extraction efficiencies of the fatty acids were compared. The developed method was used to investigate the fatty acid profiles in five types of rice (Daohuaxiang, Jixing, Jinlangzi, Nongda, and Zhuangyuan). The fatty acid profile in Daohuaxiang rice was different from that in the other four species. The fatty acids in Jinlangzi and Nongda rice were markedly different, while those in Jixing and Jinlangzi rice were similar. The method is simple, stable and accurate, and it can provide basic data for the improvement of rice quality and nutritional value.
- gas chromatography-mass spectrometry (GC-MS),
- pseudotargeted metabolomics,
- fatty acids,
- rice
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