Citation: WEI Minzhi, WAN Jianchun, ZHANG Fusheng, PAN Hua. Determination of 3-methyl-quinoxaline-2-carboxylic acid residue in pork by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2019, 37(10): 1059-1063. doi: 10.3724/SP.J.1123.2019.04050 shu

Determination of 3-methyl-quinoxaline-2-carboxylic acid residue in pork by high performance liquid chromatography-tandem mass spectrometry

1.
Test Center for Agriculture Product Quality and Safety of Jiangxi Province, Nanchang 330012, China;
2.
Jiangxi Food Inspection and Testing Research Institute, Nanchang 330001, China

Received Date: 30 April 2019

Fund Project: Key Science and Technology Program Project of Jiangxi Province (No. 20181BBF60027).

A method was developed for the simultaneous quantitative and confirmatory determination of 3-methylquinoxaline-2-carboxylic acid (MQCA) residues in pork by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The sample was hydrolyzed with 0.3 mol/L hydrochloric acid solution, and MQCA was extracted by water bath oscillation. MQCA was first extracted with acetonitrile and ethyl acetate and then re-extracted from the extraction solution with 0.1 mol/L sodium hydroxide solution. Then MQCA was purified by an anion exchange solid phase extraction column. The chromatographic separation was performed using an Agilent Eclipse Plus C18 column (50 mm×3.0 mm, 1.8 μm). The quantitative analysis was performed by the matrix matching addition method. The correlation coefficient of MQCA in the range of 1.0-50 μg/L was greater than 0.99. The recovery was 90.5%-119.6% at the spiked levels of 0.5, 1.0 and 5.0 μg/kg, and the relative standard deviation was 3.14%-4.22%. The method can be used for the rapid quantitative determination of 3-methylquinoxaline-2-carboxylic acid residues in pork.
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沈阳化工大学材料科学与工程学院沈阳 110142