Citation: XIAO Yong, WU Haizhi, YUAN Liejiang, TANG Jiwang, WANG Shuxia, WANG Xiu, DENG Hang, WU Lin. Determination of chlormequat chloride residues in animal derived foods by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2020, 38(2): 218-223. doi: 10.3724/SP.J.1123.2019.04036 shu

Determination of chlormequat chloride residues in animal derived foods by high performance liquid chromatography-tandem mass spectrometry

Hunan Province Produce Commodity Quality Supervision Testing & Research Institute, Changsha 410007, China

Corresponding author: XIAO Yong, xy882008@163.com
Received Date: 29 April 2019

A method for the determination of chlormequat chloride (CCC) residues in animal derived foods by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The samples were extracted with acetonitrile containing 1% (v/v) acetic acid and defatted with n-hexane, followed by clean-up on a cationic solid phase extraction column. The analytes were separated on a Venusil MP C18(2) column (150 mm×2.1 mm, 3 μ m) under a gradient elution program using acetonitrile and 0.1% (v/v) formic acid aqueous solution as the mobile phases. Then, the analytes were detected by tandem mass spectrometry using a positive electrospray ionization (ESI⁺) source in the multiple reaction monitoring (MRM) mode. Matrix-matched internal standard calibration curves were used for quantitative analysis. The calibration curves showed good linearity in the range of 0.200-500 μ g/L for CCC, with correlation coefficients (r²) no less than 0.9993. The limit of quantification (LOQ) of the method was 0.500 μ g/kg. The average recoveries of CCC in pork, beef, mutton, chicken, egg, pig kidney, beef liver, sheep kidney, chicken liver and milk matrices at spiked levels of 0.500-500 μ g/kg were 93.4%-101%, and the relative standard deviations were 2.3%-8.0%. The method has less matrix interference, with high sensitivity, accuracy and reliability, and it is suitable for the quantitative detection of CCC residues in animal derived foods.
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