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Citation: LIU Tong, WANG Yujiao, WANG Xiujuan, CUI Dongwei, ZHANG Feng. Simultaneous determination of nine flavor compounds in pasteurized milk by gas chromatography- triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2019, 37(11): 1215-1220. doi: 10.3724/SP.J.1123.2019.04033 shu

Simultaneous determination of nine flavor compounds in pasteurized milk by gas chromatography- triple quadrupole mass spectrometry

LIU Tong ^1, ,
WANG Yujiao ^1,2 ,
WANG Xiujuan ¹ ,
CUI Dongwei ^1,3 ,
ZHANG Feng ¹

1.
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, China;
2.
College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300000, China;
3.
School of Pharmacy, China Medical University, Shenyang 110122, China

Received Date: 21 April 2019

Fund Project: National Key Research and Development Program (Nos. 2017YFC1601600, 2018YFC1603606)

A gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) method was established for the simultaneous determination of nine flavor compounds (hydrocoumarin, vanillin, coumarin, ethyl vanillin, methyl vanillin, 7-methylcoumarin, 7-methyoxycoumarin, 7-ethoxy-4-mrthyl coumarin, pyranocoumarin) in pasteurized milk. The samples were extracted with ethanol and subjected to whirlpool oscillation. After centrifugation, the supernatant was filtered through a membrane, and the components were separated on a DB-5MS column. The nine analytes were detected by GC-MS/MS in the multiple reaction monitoring (MRM) mode and quantified by a matrix-matched external standard method. Good linearities in the range of 1-200 μg/L were observed for the nine flavor compounds. The linear correlation coefficients (R²) were greater than 0.997. The limits of detection and limits of quantification were 0.002-0.1 μg/kg and 0.001-2 μg/kg, respectively. The average recoveries were 90.3%-110.6%. Both the intra-day and inter-day RSDs were less than 10%. The developed method is simple, rapid, accurate and sensitive, and can be used for the simultaneous determination of the nine flavor compounds in pasteurized milk.
- gas chromatography-tandem mass spectrometry (GC-MS/MS),
- flavor compounds,
- pasteurized milk
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