Citation: ZHOU Ye, LIU Zheyi, WANG Fangjun. Progress in structural proteomics research[J]. Chinese Journal of Chromatography, ;2019, 37(8): 788-797. doi: 10.3724/SP.J.1123.2019.04015 shu

Progress in structural proteomics research

ZHOU Ye ^1,2, ,
LIU Zheyi ¹ ,
WANG Fangjun ¹

1.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 8 April 2019

Fund Project: National Natural Science Foundation of China (No. 91853101, 21675152).

The structures of proteins are directly related to their biological functions. The regulation of protein functions mainly depends on the dynamic regulation of their conformations and interactions. The study of protein structures and functions plays an essential role in the field of life science, which is also an important direction of proteomics development. The emergence of proteomics has led to major technological advances in mass spectrometry (MS). These advancements have not only benefitted MS-based high-throughput proteomics but have also increased the impact of MS in the fields of structural and molecular biology. This review briefly describes the principles, progresses and applications of the MS-based structural proteomics methods in recent years, including the native MS analysis of active proteins, limited proteolysis, chemical cross-linking, hydrogen-deuterium exchange, covalent labeling, and thermal proteome profiling. Finally, future development of the structural proteomics was summarized and prospected.
- structural proteomics,
- mass spectrometry (MS),
- protein interaction,
- review
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