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Citation: DU Yingying, LIU Xiangzhen, YE Meijun, XU Jianfeng, GAO Haiyan, ZHENG Guojian. Detection of free amino acids in tea using ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2019, 37(6): 597-604. doi: 10.3724/SP.J.1123.2018.12041 shu

Detection of free amino acids in tea using ultra-high performance liquid chromatography-tandem mass spectrometry

Hangzhou Tea Research Institute, CHINA COOP, China National Center of Quality Supervision and Inspection of Tea, Hangzhou 310016, China

Received Date: 2 April 2019

Fund Project: National Key Research and Development Plan (No. 2018YFF0214203)

To efficiently and quickly detect free amino acid components in tea, a method using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed. With the optimization of mass spectrometry, chromatographic conditions, and amino-acid extraction conditions, a total of 20 free amino acids were identified using 5 mmol/L ammonium acetate aqueous solution containing 0.2% (v/v) formic acid and methanol as mobile phases for gradient elution and detected by electrospray ionization (ESI) and positive-ion scanning. The results showed that all calibration curves expressed good linearities. Theanine (Thea), Arg, Asn, and Asp were in the range of 50-500 μg/L. The other amino acids were in the range of 10-250 μg/L with all correlation coefficients ≥ 0.99. The average recoveries were between 92.3% and 109.2%. The relative standard deviation (RSDs) were between 2.00% and 9.88%. The limits of detection (LODs) were 0.001-0.011 mg/L, and the limits of quantification (LOQs) were 0.010-0.053 mg/L. The method is sensitive, accurate, and has good repeatability and stability. The method can effectively detect 20 types of amino acids and amino components in tea leaves from the samples of green tea, white tea, and black tea.
- ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- free amino acids,
- tea,
- extraction method
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