Citation: JIANG Dandan, MA Jiutong, JIA Qiong. Preparation of polyoxometalate-chitosan magnetic composite for the enrichment of phosphopeptides[J]. Chinese Journal of Chromatography, ;2019, 37(3): 247-251. doi: 10.3724/SP.J.1123.2018.10044 shu

Preparation of polyoxometalate-chitosan magnetic composite for the enrichment of phosphopeptides

College of Chemistry, Jilin University, Changchun 130012, China

Received Date: 31 October 2018

Fund Project: Open Project of State Key Laboratory of Supramolecular Structure and Materials, Jilin University (No.sklssm201815).

A method for the enrichment of phosphopeptides based on magnetic nanoparticles coated with polyoxometalate (POM) was developed. A layer-by-layer assembly technique was used to prepare magnetic nanoparticles (MNPs) coated with polyoxometalate and chitosan. The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides. β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique. After the enrichment step, the limit of detection was determined to be 0.02 fmol. The prepared POM-based MNPs thus exhibit significant potential in the detection of low-abundance phosphoproteins.
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
1. [1]
  Feng Lin , Zhongxin Jin , Caiying Li , Cheng Shao , Yang Xu , Fangze Li , Siqi Liu , Ruining Gu . Preparation and Electrochemical Properties of Nickel Foam-Supported Ni(OH)₂-NiMoO₄ Electrode Material. University Chemistry, 2025, 40(10): 225-232. doi: 10.12461/PKU.DXHX202412017
2. [2]
  Xinyu Miao , Hao Yang , Jie He , Jing Wang , Zhiliang Jin . Adjusting the electronic structure of Keggin-type polyoxometalates to construct S-scheme heterojunction for photocatalytic hydrogen evolution. Acta Physico-Chimica Sinica, 2025, 41(6): 100051-0. doi: 10.1016/j.actphy.2025.100051
3. [3]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
4. [4]
  Zhengzheng LIU , Pengyun ZHANG , Chengri WANG , Shengli HUANG , Guoyu YANG . Synthesis, structure, and electrochemical properties of a sandwich-type {Co₆}-cluster-added germanotungstate. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1173-1179. doi: 10.11862/CJIC.20240039
5. [5]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
6. [6]
  Jie WEI , Qing ZHOU , Dandan DING , Xiang JING , Fei LI . Photothermal toxicity of Prussian blue nanoparticles to cervical cancer cells. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2345-2357. doi: 10.11862/CJIC.20240435
7. [7]
  Gaopeng Liu , Lina Li , Bin Wang , Ningjie Shan , Jintao Dong , Mengxia Ji , Wenshuai Zhu , Paul K. Chu , Jiexiang Xia , Huaming Li . Construction of Bi Nanoparticles Loaded BiOCl Nanosheets Ohmic Junction for Photocatalytic CO₂ Reduction. Acta Physico-Chimica Sinica, 2024, 40(7): 2306041-0. doi: 10.3866/PKU.WHXB202306041
8. [8]
  Zuoyong Li , Haoxiang Tu , Mingwei Ding , Meijun Liu , Ting Yang . Innovative Teaching Reform Study on the Synthesis of Silver Nanoparticles Based on Machine Learning and Microfluidic Technology. University Chemistry, 2026, 41(1): 64-75. doi: 10.12461/PKU.DXHX202505088
9. [9]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
10. [10]
  Kezhen Qi , Bei Cheng , Kaiqiang Xu . Ultrafast interfacial charge transfer promoted by the LSPR of Au nanoparticles for photocatalytic H₂ evolution. Acta Physico-Chimica Sinica, 2026, 42(3): 100205-0. doi: 10.1016/j.actphy.2025.100205
11. [11]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
12. [12]
  Siyu HOU , Weiyao LI , Jiadong LIU , Fei WANG , Wensi LIU , Jing YANG , Ying ZHANG . Preparation and catalytic performance of magnetic nano iron oxide by oxidation co-precipitation method. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1577-1582. doi: 10.11862/CJIC.20230469
13. [13]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
14. [14]
  Qianqian Zhong , Yucui Hao , Guotao Yu , Lijuan Zhao , Jingfu Wang , Jian Liu , Xiaohua Ren . Comprehensive Experimental Design for the Preparation of the Magnetic Adsorbent Based on Enteromorpha Prolifera and Its Utilization in the Purification of Heavy Metal Ions Wastewater. University Chemistry, 2024, 39(8): 184-190. doi: 10.3866/PKU.DXHX202312013
15. [15]
  Dingwen CHEN , Siheng YANG , Haiyan FU , Hua CHEN , Xueli ZHENG , Weichao XUE , Jiaqi XU , Ruixiang LI . NiOOH-mediated synthesis of gold nanoaggregates for electrocatalytic performance for selective oxidation of glycerol to glycolate. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2317-2326. doi: 10.11862/CJIC.20250053
16. [16]
  Xiaofei Liu , He Wang , Li Tao , Weimin Ren , Xiaobing Lu , Wenzhen Zhang . Electrocarboxylation of Benzylic Phosphates and Phosphinates with Carbon Dioxide. Acta Physico-Chimica Sinica, 2024, 40(9): 2307008-0. doi: 10.3866/PKU.WHXB202307008
17. [17]
  Guimin ZHANG , Wenjuan MA , Wenqiang DING , Zhengyi FU . Synthesis and catalytic properties of hollow AgPd bimetallic nanospheres. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 963-971. doi: 10.11862/CJIC.20230293
18. [18]
  Tingting XU , Wenjing ZHANG , Yongbo SONG . Research advances of atomic precision coinage metal nanoclusters in tumor therapy. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2275-2285. doi: 10.11862/CJIC.20240229
19. [19]
  Zeyu Liu , Wenze Huang , Yang Xiao , Jundong Zhang , Weijin Kong , Peng Wu , Chenzi Zhao , Aibing Chen , Qiang Zhang . Nanocomposite Current Collectors for Anode-Free All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2305040-0. doi: 10.3866/PKU.WHXB202305040
20. [20]
  Zhengyu Zhou , Huiqin Yao , Youlin Wu , Teng Li , Noritatsu Tsubaki , Zhiliang Jin . Synergistic Effect of Cu-Graphdiyne/Transition Bimetallic Tungstate Formed S-Scheme Heterojunction for Enhanced Photocatalytic Hydrogen Evolution. Acta Physico-Chimica Sinica, 2024, 40(10): 2312010-0. doi: 10.3866/PKU.WHXB202312010

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(657)
HTML views(72)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142