Citation: GONG Ruize, WANG Yanhua, QI Yuli, CHEN Lixue, LI Shanshan, SUN Yinshi. Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum[J]. Chinese Journal of Chromatography, ;2019, 37(2): 194-200. doi: 10.3724/SP.J.1123.2018.10042 shu

Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum

GONG Ruize ^1, ,
WANG Yanhua ^1,2 ,
QI Yuli ^1,2 ,
CHEN Lixue ^1,2 ,
LI Shanshan ¹ ,
SUN Yinshi ¹

1.
Institute of Special Wild Economic Animal and Plant, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
2.
College of Traditional Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China

Received Date: 30 October 2018

Fund Project: National Key Research and Development Program of China (Nos. 2018YFC1706604, 2018YFC1706605)

The water-soluble polysaccharides in antler velvet were extracted by water extraction and alcohol precipitation. The content was determined by a phenol-sulfuric acid colorimetry. After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC). The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively. The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively. Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods. For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood. For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces. This study provides a theoretical reference for velvet processing and product development.
- ultra high performance liquid chromatography (UPLC),
- comparative analysis,
- processing methods,
- parts,
- water-soluble polysaccharide,
- monosaccharide composition,
- Cervi Cornu Pantotrichum
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