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Citation: WEN Jiaxin, WU Fengdan, CAO Yajing, HE Jiawen, HUANG Zhiye, HUANG Yanting, TAN Wanqing, LUO Wenjing, LAI Yuhong. Determination of chloramphenicol in propolis and propolis-derived dietary supplements by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2018, 36(12): 1284-1289. doi: 10.3724/SP.J.1123.2018.08012 shu

Determination of chloramphenicol in propolis and propolis-derived dietary supplements by high performance liquid chromatography-tandem mass spectrometry

Guangdong Institute for Drug Control, Guangzhou 510663, China

Received Date: 11 August 2018

Fund Project: Guangdong Science and Technology Project (No. 2013B090200059).

A method for determining chloramphenicol (CAP) in both propolis and propolis-derived dietary supplements was developed by utilizing high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The flavones in the samples were removed with a lead acetate solution and ammonia, and the fat-soluble interferences, such as beewax and vegetable oils, were removed with n-hexane after the sample dissolved in ethanol. Tert-butyl methyl ether was used as the back-extraction solvent to reduce co-extracting compounds, such as polyethylene glycol 400 (PEG 400) and glycerol, which are common adjuvants of dietary supplements, and some polar interferences. CAP was detected by HPLC-MS/MS and quantified by the internal standard method. The calibration curve showed a good linearity in the range of 0.20-50.0 μg/L. The limits of detection and the limits of quantification were 0.03 and 0.1 μg/kg, respectively. The recoveries in four different matrices at three spiked levels were in the 86.0%-114.4% range with the relative standard deviations from 0.3% to 4.9%. With the advantages of excellent universality, ease of operation, high sensitivity, and strong anti-interference capability, the proposed method was suitable for the determination of CAP in both propolis and propolis-derived dietary supplements.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- chloramphenicol,
- propolis,
- health food,
- soft capsule,
- hard capsule
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