Citation: WANG Ping, LI Mengqi, ZHAO Liyuan, YANG Yi, DING Xiaojing. Uniform and orthogonal designs on experimental design and optimization of micellar electrokinetic capillary chromatographic method for the simultaneous analysis of three nucleoside antivirus drugs in disinfectant and anti/bacteriostatic products[J]. Chinese Journal of Chromatography, ;2018, 36(9): 931-937. doi: 10.3724/SP.J.1123.2018.04003 shu

Uniform and orthogonal designs on experimental design and optimization of micellar electrokinetic capillary chromatographic method for the simultaneous analysis of three nucleoside antivirus drugs in disinfectant and anti/bacteriostatic products

WANG Ping ^1,2, ,
LI Mengqi ³ ,
ZHAO Liyuan ³ ,
YANG Yi ³ ,
DING Xiaojing ^1,2

1.
Beijing Center for Disease Control and Prevention, Beijing 100013, China;
2.
Beijing Research Center for Preventive Medicine, Beijing 100013, China;
3.
College of Science, Beijing University of Chemical Technology, Beijing 100029, China

Received Date: 1 April 2018

Fund Project: Project for Department of Law and Legislation, National Health and Family Planning Commission of the Peoples's Republic of China (No. 20171704).

To prevent the abuse of nucleoside-based antiviral drugs in disinfectant and anti/bacteriostatic products, a new micellar electrokinetic chromatographic (MEKC) method for the simultaneous determination of three nucleoside antiviral drugs (ganciclovir, acyclovir and penciclovir) was established. Three factors and seven levels uniform design and four factors and four levels orthogonal design were used to optimize the concentration of sodium dodecyl sulfate (SDS), sodium dihydrogen phosphate (NaH₂PO₄) and sodium borate (Na₂B₄O₇) in the running buffer. The three nucleoside antiviral drugs were separated from each other in the shortest time to obtain effective separation through uniform and orthogonal designs on experimental design and optimization. The separation was performed on an uncoated fused-silica capillary with 50 μ m i. d. and a total length of 30.2 cm (effective length:20 cm). An optimized buffer solution consisting of 25 mmol/L NaH₂PO₄, 10 mmol/L Na₂B₄O₇ (pH 7.41) and 140 mmol/L dodecyl sulfate sodium was used for separation. The applied voltage was 10 kV, the injected pressure and time was at 0.003 Pa for 4 s. The wavelength of the detection was 250 nm. Under the optimum conditions, the corrected area and the concentration had good linearity. The correlation coefficients (r) were not less than 0.9995. The limits of detection were all 2.0 mg/kg. The limits of quantitation were all 7.0 mg/kg. The relative recoveries ranging from 85.4% to 104.1% with RSDs all lower than 8.0%. It showed that the method was suitable for the detection of ganciclovir, acyclovir and penciclovir in disinfectant and anti/bacteriostat products with simplicity and rapidity.
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