Home

Citation: REN Ping, LIU Jing, LIN Risheng, LIU Yang, HUANG Meisha, HU Sheng, XU Youchun, LI Caixia. Construction of a microfluidic chip-based 72 plex single nucleotide polymorphisms ancestry inference system[J]. Chinese Journal of Chromatography, ;2018, 36(7): 599-607. doi: 10.3724/SP.J.1123.2018.01003 shu

Construction of a microfluidic chip-based 72 plex single nucleotide polymorphisms ancestry inference system

REN Ping ^1,2,3, ,
LIU Jing ² ,
LIN Risheng ⁴ ,
LIU Yang ¹ ,
HUANG Meisha ¹ ,
HU Sheng ² ,
XU Youchun ³ ,
LI Caixia ^1,2

1.
Shanxi Medical University, Taiyuan 036000, China;
2.
Beijing Engineering Research Center of Crime Scene Evidence Examination, National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Beijing 100038, China;
3.
Department of Biomedical Engineering, Tsinghua University, Beijing 100084, China;
4.
Inner Mongolia Xilingol League Public Security Bureau Criminal Police Detachment, Xilinhot 026000, China

Received Date: 3 January 2018

Fund Project: National Key Plan(No. 2017YFC0803501)

The aim was to develop an autosomal single nucleotide polymorphism (SNP) multiplex detection chip system for the inference of the ancestry of unknown individuals. On the basis of the 74-ancestry informative SNP (AISNP) panel screened by our group, a multiplex SNP amplification and detection system, based on a microfluidic chip of competitive allele-specific polymerase chain reaction (PCR), was constructed. The allele-specific primers of each SNP were positioned into a single well on the microfluidic chip and each chip included 112 wells. For detection of SNPs, the chip was placed on the plate thermocycler to perform PCR. The fluorescence signal of the reaction in each well was detected by a confocal laser scanning instrument to evaluate the SNP genotypes of each sample. Fifty-two samples were used for SNP typing. The accuracy of typing results was 100%. Taking 3628 samples of 57 populations as the reference population database, the ancestry of 20 samples was inferred. The results were consistent with the actual sources of the samples. The autosomal 72-SNP microfluidic chip system established can accurately detect SNPs. The ancestry of tested individuals can be accurately inferred from the reference database.
- ancestry inference,
- single nucleotide polymorphism complex system,
- microfluidic chip
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
1. [1]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
2. [2]
  Zuoyong Li , Haoxiang Tu , Mingwei Ding , Meijun Liu , Ting Yang . Innovative Teaching Reform Study on the Synthesis of Silver Nanoparticles Based on Machine Learning and Microfluidic Technology. University Chemistry, 2026, 41(1): 64-75. doi: 10.12461/PKU.DXHX202505088
3. [3]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
4. [4]
  Rui Gao , Ying Zhou , Yifan Hu , Siyuan Chen , Shouhong Xu , Qianfu Luo , Wenqing Zhang . Design, Synthesis and Performance Experiment of Novel Photoswitchable Hybrid Tetraarylethenes. University Chemistry, 2024, 39(5): 125-133. doi: 10.3866/PKU.DXHX202310050
5. [5]
  Qin Tu , Anju Tao , Tongtong Ma , Jinyi Wang . Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip. University Chemistry, 2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062
6. [6]
  Zhibei Qu , Changxin Wang , Lei Li , Jiaze Li , Jun Zhang . Organoid-on-a-Chip for Drug Screening and the Inherent Biochemistry Principles. University Chemistry, 2024, 39(7): 278-286. doi: 10.3866/PKU.DXHX202311039
7. [7]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055
8. [8]
  Keying Qu , Jie Li , Ziqiu Lai , Kai Chen . Unveiling the Mystery of Chirality from Tartaric Acid. University Chemistry, 2024, 39(9): 369-378. doi: 10.12461/PKU.DXHX202310091
9. [9]
  Yongmei Chen , Lidan Zhang , Shunlai Li , Chunting Zhang , Meng Cui , Qinghong Xu , Lan Jin , Chunchuang Li , Zhi Lv . Development of a National First-Class Course of “University Chemistry Experiment” Based on MOOCs. University Chemistry, 2024, 39(7): 8-12. doi: 10.3866/PKU.DXHX202404017
10. [10]
  Fan Yu , Aihua Li , Yun Liu , Tianrong Zhu , Liang Wang , Junhui Xu , Yazhen Wang . Exploration and Practice in Developing a Premier Course in Inorganic and Analytical Chemistry. University Chemistry, 2024, 39(8): 36-43. doi: 10.3866/PKU.DXHX202312037
11. [11]
  Shuyong Zhang , Wenfeng Jiang , Changsheng Lu , Genrong Qiang , Yongmei Liu , Xiangyang Tang , Dongcheng Liu , Lili Zhang . Suggestions on Construction and Evaluation Standards for First-Class Chemical Experiment Teaching. University Chemistry, 2025, 40(5): 9-14. doi: 10.12461/PKU.DXHX202502114
12. [12]
  Zijian Jiang , Yuang Liu , Yijian Zong , Yong Fan , Wanchun Zhu , Yupeng Guo . Preparation of Nano Zinc Oxide by Microemulsion Method and Study on Its Photocatalytic Activity. University Chemistry, 2024, 39(5): 266-273. doi: 10.3866/PKU.DXHX202311101
13. [13]
  Xiaojun Liu , Lang Qin , Yanlei Yu . Dynamic Manipulation of Photonic Bandgaps in Cholesteric Liquid Crystal Microdroplets for Applications. Acta Physico-Chimica Sinica, 2024, 40(5): 2305018-0. doi: 10.3866/PKU.WHXB202305018
14. [14]
  Xiaoyi Sun , Duohang Bi , Hankun Qiao , Yijing Liu , Jintao Zhu . Painless Injection: Microneedles Revolutionizing Beauty and Health Brought. University Chemistry, 2025, 40(10): 166-174. doi: 10.12461/PKU.DXHX202411006
15. [15]
  Ruiying WANG , Hui WANG , Fenglan CHAI , Zhinan ZUO , Benlai WU . Three-dimensional homochiral Eu(Ⅲ) coordination polymer and its amino acid configuration recognition. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 877-884. doi: 10.11862/CJIC.20250052
16. [16]
  Jingxuan Zhang , Weihao Jiang , Siyuan Zhang , Hongye Tian , Ziye Huang , Lin Huang , Qikun Wu , Jing Yang , Yibin Jiang , Cheng Wang . Automation and AI-Assisted Investigation of the Chemical Reactivity of Sulfosalicylic Acid. University Chemistry, 2026, 41(1): 332-345. doi: 10.12461/PKU.DXHX202505108
17. [17]
  Ke Li , Chuang Liu , Jingping Li , Guohong Wang , Kai Wang . Architecting Inorganic/Organic S-Scheme Heterojunction of Bi₄Ti₃O₁₂ Coupling with g-C₃N₄ for Photocatalytic H₂O₂ Production from Pure Water. Acta Physico-Chimica Sinica, 2024, 40(11): 2403009-0. doi: 10.3866/PKU.WHXB202403009
18. [18]
  Hui Xiong , Yan Wang , Rongxian Bai , Yongqi Wu , Chengmei Liu , Yuefa Gong , Jian Zhang . Development of a Compound Talent Training System Based on Virtual Technology: a Case Study of Chemical Unit and Process Simulation Practices. University Chemistry, 2024, 39(10): 314-317. doi: 10.12461/PKU.DXHX202405071
19. [19]
  Nana Wang , Gaosheng Zhang , Huosheng Li , Tangfu Xiao . Discussion on the Teaching Reform of Environmental Functional Materials within the Context of “Double First-Class” Initiative: Emphasizing the Integration of Industry, Academia, Research, and Application. University Chemistry, 2024, 39(6): 137-144. doi: 10.3866/PKU.DXHX202312010
20. [20]
  Yue Zhang , Chengxin Shang , Caihong Zhang , Jikai Pei , Lihong Shi , Pengfei Gao , Zhiqi Jia , Songbai Wang , Junsheng Hao , Guomei Zhang , Wei Guo . Reforms and Practices of the Chemistry Experimental Teaching Demonstration Center under the Background of National First-Class Major Construction. University Chemistry, 2024, 39(7): 207-210. doi: 10.12461/PKU.DXHX202405134

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(622)
HTML views(86)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return