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Citation: FU Hui, HU Xiaojian, CHEN Xi, LIN Shaobin. Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2018, 36(5): 487-492. doi: 10.3724/SP.J.1123.2017.12029 shu

Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry

National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China

Received Date: 28 December 2017

Fund Project: Commonweal and Environmental Protection Project of the Ministry of Environmental Protection of the People's Republic of China (No. 201309045).

An efficient and accurate method was developed for the simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine. A 10.0-mL urine sample was hydrolyzed by enzyme and extracted using solid phase extraction. The metabolites were separated on an Acquity UPLC^®HSS T3 column (100 mm×2.1 mm, 1.8 μm) with a mobile phase comprising methanol and water. The analytes were detected by electrospray ionization (ESI)-MS/MS in negative mode and multiple reaction monitoring (MRM) mode. The linear ranges of the 12 metabolites were 0.04-20.0 μg/L with correlation coefficients above 0.99. The detection limits were in the range of 0.01-0.41 μg/L and the recoveries were 80.0%-105%. The intra-group relative standard deviations (RSDs) were less than 9.12% and the inter-group RSDs were less than 19.7%. This method was applied to the analysis of 100 urine samples and the results showed that the method was simple, sensitive and selective.
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