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Citation: ZHU Jingbo, LI Wenyu, XIAO Wei, DING Yan, HUANG Wenzhe, TU Pengfei, WANG Yonghua. Separation of lignans from Schisandra chinensis by two-dimensional reversed-phase liquid chromatography[J]. Chinese Journal of Chromatography, ;2018, 36(5): 464-473. doi: 10.3724/SP.J.1123.2017.12025 shu

Separation of lignans from Schisandra chinensis by two-dimensional reversed-phase liquid chromatography

ZHU Jingbo ^1,2, ,
LI Wenyu ¹ ,
XIAO Wei ² ,
DING Yan ¹ ,
HUANG Wenzhe ² ,
TU Pengfei ^2,3 ,
WANG Yonghua ^2,4

1.
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China;
2.
Jiangsu Kanion Pharmaceutical Corporation Limited, Lianyungang 222001, China;
3.
Peking University, Beijing 100871, China;
4.
Northwest Agriculture and Forestry University, Yangling 712100, China

Received Date: 27 December 2017

Fund Project: Project of Joint Fund of the National Natural Science Foundation of China (No. U1603285)

A two-dimensional reversed-phase liquid chromatography (2D-RPLC) method was established to separate lignan components and pure compounds from Schisandra chinensis. This method was based on the modules of separation-enrichment and self-developed with independent intellectual property rights. First, a C₁₈ column (250 mm×4.6 mm, 5 μm) was used as the analytical column. A water-methanol mixture was selected as the mobile phase. Separation resolution and retention time were selected as indexes. Gradient chromatography of the lignan extracts was repeated four times and the information was input into XTool, a chromatographic separation software. The conditions for the first-and second-dimensional chromatographic separation were optimized by XTool. Then, linear amplification was used for preparative chromatography. C₁₈ column (250 mm×30 mm, 10 μm) was selected as the first-and second-dimensional separation column. The C₁₈ columns (80 mm×30 mm, 10 μm) were selected as enriching columns, and water was chosen as the diluent solution. Lignans from Schisandra chinensis were separated and purified by 2D-RPLC. Finally, nine lignan components were obtained by the first-dimensional separation. Totally, 20 high-purity compounds, containing six monomers, were obtained by the second-dimensional separation. Experimental results showed that this method has good reproducibility. This method thus allows for the systematic separation of lignans from Schisandra chinensis.
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