Citation: GAO Jie, CHEN Dawei, ZHAO Yunfeng. Determination of carbendazim and thiabendazole in wine and beer by ultra high performance liquid chromatography-high resolution mass spectrometry coupled with dispersive micro solid-phase extraction[J]. Chinese Journal of Chromatography, ;2018, 36(2): 143-149. doi: 10.3724/SP.J.1123.2017.10018 shu

Determination of carbendazim and thiabendazole in wine and beer by ultra high performance liquid chromatography-high resolution mass spectrometry coupled with dispersive micro solid-phase extraction

China National Centre for Food Safety Risk Assessment, Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China

Received Date: 17 October 2017

Fund Project: China Food Safety Talent Competency Development Initiative:China National Center for Food Safety Risk Assessment (CFSA)523 Program.

A rapid method was established for the determination of carbendazim and thiabendazole in wine and beer by ultra high performance liquid chromatography-Q Orbitrap high resolution mass spectrometry and dispersive micro solid-phase extraction (DMSPE) based on a strong cation exchange adsorbent (PCX). For the pretreatment method, the amount of PCX, the volume percentage of acetonitrile, the volume percentage of ammonium hydroxide and the volume of eluent were optimized. The analytes were separated on a BEH C₁₈ column (50 mm×2.1 mm, 1.7 μm) and detected using targeted single ion monitoring-data dependent tandem mass spectrometry (tSIM-ddMS²) scan mode. The method showed a good linearity within a certain concentration ranges with correlation coefficients R² ≥ 0.9999. The detection limits of carbendazim and thiabendazole in wine and beer were 0.02 and 0.01 μg/L, and the quantification limits were 0.06 and 0.03 μg/L. The average recoveries of carbendazim and thiabendazole at the spiked levels of 0.1, 1.0 and 100 μg/L were in the ranges of 95.6%-110.2% and 87.5%-102.8%, with intra-day precision (RSD_r) ranges from 1.8%-5.2% and 1.3%-4.8%, and inter-day precision (RSD_R) ranges from 4.3%-8.7% and 4.8%-9.4%. The method is rapid, simple and sensitive for the simultaneous determination of carbendazim and thiabendazole in wine and beer.
- dispersive micro solid-phase extraction (DMSPE),
- ultra high performance liquid chromatography (UHPLC),
- high resolution mass spectrometry (HRMS),
- carbendazim,
- thiabendazole,
- wine,
- beer
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