Citation: GU Yi, GUO Ming, LÜ Da, HOU Ping, YIN Xinxin. Investigation on the interaction between pentadecafluorooctanoic acid and human serum albumin by capillary electrophoresis[J]. Chinese Journal of Chromatography, ;2018, 36(1): 69-77. doi: 10.3724/SP.J.1123.2017.09041 shu

Investigation on the interaction between pentadecafluorooctanoic acid and human serum albumin by capillary electrophoresis

GU Yi ^1, ,
GUO Ming ^1,2 ,
LÜ Da ¹ ,
HOU Ping ¹ ,
YIN Xinxin ²

1.
School of Forestry and Bio-technology, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China;
2.
School of Science, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China

Received Date: 26 September 2017

Fund Project: National Natural Science Foundation of China (No. 20877072).

Capillary electrophoresis (CE) has been used to establish the analytical method of interaction between pentadecafluorooctanoic acid (PFOA) and human serum albumin (HSA). Under the physiological conditions, the interaction model of PFOA and HSA were constructed. Mobility method, plug-plug kinetic (PPK) method and simplified Hummel-Dreyer method were used to determine the interaction between derivatives and HSA. Non-linear regression, Scatchard equation and Klotz equation were adopted to obtain the interaction parameters. The results showed that all the three methods can be used to analyze the interaction of PFOA-HSA system. According to the interaction parameters, the most suitable CE method is simplified Hummel-Dreyer method while the most suitable theoretical equation is non-linear regression. The binding parameters indicated that the interaction of PFOA-HSA system has only one type of binding sites and the binding is stable. The research results have illustrated the interaction between HSA and PFOA, and provided a beneficial reference for in-depth research of the toxic mechanism of PFOA.
- capillary electrophoresis (CE),
- pentadecafluorooctanoic acid (PFOA),
- human serum albumin (HSA),
- binding parameters
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