Citation: HOU Kun, WATANABE Masaki, WANG Dali, HAN Jing, LI Yonglu, HUO Hong. Determination of 6-benzylaminopurine in edible mushrooms by solid liquid extraction with low-temperature partition and high performance liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2018, 36(1): 12-16. doi: 10.3724/SP.J.1123.2017.09028 shu

Determination of 6-benzylaminopurine in edible mushrooms by solid liquid extraction with low-temperature partition and high performance liquid chromatography-mass spectrometry

HOU Kun ^1, ,
WATANABE Masaki ² ,
WANG Dali ³ ,
HAN Jing ¹ ,
LI Yonglu ¹ ,
HUO Hong ⁴

1.
Hitachi Instruments(Dalian) Co., Ltd., Dalian 116033, China;
2.
Hitachi High-Tech Science Corporation, Ibaraki 3120033, Japan;
3.
Dalian Children's Hospital, Dalian 116012, China;
4.
Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, China

Received Date: 17 September 2017

A simple method based on solid liquid extraction with low-temperature partition (SLE-LTP) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed for the determination of 6-benzylaminopurine (6-BA) in edible mushrooms. The samples were extracted with acetonitrile followed by low-temperature partition at -30℃ for 4 h. The separation was performed on a Hitachi LaChrom C18 column (250 mm×4.6 mm, 5 μm) with 0.02 mol/L ammonium acetate (containing 0.1% (v/v) glacial acetic acid)-methanol (6:4, v/v) as the mobile phases with isocratic elution. The compound was detected in positive electrospray ionization mode, and quantified by external standard method. The results showed that the limit of detection (LOD, S/N>3) for the analyte was 0.006 mg/kg, and the limit of quantification (LOQ, S/N>10) was 0.02 mg/kg. The calibration curve showed good linear in the range of 0.05-2.0 mg/L, and the correlation coefficient (r²) was 1.0000. The spiked recoveries were between 81.3% and 93.7% with the relative standard deviations (RSDs, n=6) of 0.7%-2.4% at the spiked levels of 0.1 mg/kg and 0.5 mg/kg. The developed method is simple, reliable and can be applied for the accurate determination of 6-BA residue in edible mushrooms.
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