Citation: LI Rong, HE Chunmei, YANG Luqi, WANG Yong, ZHANG Pengjie, GAO Yongqing. Determination of 11 mycotoxins in baked foods and raw materials by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry[J]. Chinese Journal of Chromatography, ;2017, 35(8): 808-815. doi: 10.3724/SP.J.1123.2017.03035 shu

Determination of 11 mycotoxins in baked foods and raw materials by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry

LI Rong ^1, ,
HE Chunmei ^1,2 ,
YANG Luqi ^1,2 ,
WANG Yong ¹ ,
ZHANG Pengjie ¹ ,
GAO Yongqing ²

1.
Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528400, China;
2.
Guangdong Pharmaceutical University, Zhongshan 528458, China

Received Date: 27 March 2017

Fund Project: Science and Technology Planning Project of Guangdong Province of China (No. 2014A040401011)

A method for the determination of 11 mycotoxins in baked foods and raw materials by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-HRMS) is reported in this paper. The samples were extracted with 20 mL 90% (v/v) acetonitrile aqueous solution containing 1% (v/v) formic acid, and the extracts were salted out by 2.0 g MgSO₄ and 0.5 g NaCl, cleaned up by 300 mg C18. The analytes were carried out on a CORTECS C18 column (100 mm×2.1 mm, 1.6 μ m) by gradient elution with 2 mmol/L ammonium acetate with 0.1% (v/v) formic acid aqueous solution and 2 mmol/L ammonium acetate methanol with 0.1% (v/v) formic acid. The results showed that the 11 mycotoxins had good linear relationships in their respective mass concentration ranges. The correlation coefficients were not less than 0.9960 and the limits of quantitation (LOQs) were from 0.15 to 20.00 μ g/kg. The recoveries of the 11 mycotoxins in bread ranged from 64.38% to 122.61% with the relative standard deviations (RSDs) from 1.52% to 12.99% at three spiked levels (n=6). The method is demonstrated to be simple, fast, highly sensitive, reliable and it is effective to detect common mycotoxins in baked foods and raw materials.
- ultra-high performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS),
- mycotoxins,
- baked foods
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