Citation: NIU Mengna, MA Hongyan, HU Fei, WANG Shige, LIU Lu, CHANG Haizhou, HUANG Mingxian. Preparation of large-pore silica microspheres using templating method and their applications to protein separation with high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2017, 35(6): 565-571. doi: 10.3724/SP.J.1123.2017.03018 shu

Preparation of large-pore silica microspheres using templating method and their applications to protein separation with high performance liquid chromatography

Department of Chemistry, College of Science, University of Shanghai for Science and Technology, Shanghai 200093, China

Received Date: 17 March 2017

Fund Project: Science and Technology Commission of Shanghai Municipality Project (No. 14440502300)

Large-pore silica microspheres were synthesized by utilizing weak cation exchange polymer beads as templates, N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride (TMSPTMA) as a structure-directing agent, tetraethoxysilane (TEOS) as a silica precursor, and triethanolamine as a weak base catalyst. The hydrolysis and condensation of the silica precursors occurred inside the templating polymer beads yielded polymer/silica composite microspheres. After the organic polymer templates were removed in the calcination step, large-pore silica microspheres were produced. The effects of different reaction conditions on the morphology, structure and dispersibility of the formed silica microspheres were investigated. It has been shown that when the volume ratio of TMSPTMA, TEOS and triethanolamine was 1:2:2, silica microspheres with pore size range of 50-150 nm and particle size around 2 μm were obtained. The as-prepared silica microspheres were then bonded with chlorodimethyloctadecylsilane (C18), packed into a 50 mm×4.6 mm column, and evaluated for the separations of some common standard proteins and soybean isolation proteins. The results showed that the large-pore silica spheres from this work have potentials for protein separation in HPLC.
- templating method,
- structure-directing agent,
- large-pore silica microspheres,
- high performance liquid chromatography (HPLC),
- protein separation
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