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Citation: ZHANG Xin, JIANG Rui, YANG Shu, SUN Liquan, PANG Siping, LUO Aiqin. Specific recognition and detection of target proteins by new fluorescence molecularly imprinted membrane[J]. Chinese Journal of Chromatography, ;2017, 35(3): 252-254. doi: 10.3724/SP.J.1123.2016.10001 shu

Specific recognition and detection of target proteins by new fluorescence molecularly imprinted membrane

ZHANG Xin ^1,2, ,
JIANG Rui ¹ ,
YANG Shu ¹ ,
SUN Liquan ¹ ,
PANG Siping ² ,
LUO Aiqin ¹

1.
School of Life Science, Beijing Institute of Technology, Beijing 100081, China;
2.
School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China

Received Date: 4 October 2016

A new method for producing L-cysteine-capped ZnS quantum dots (QDs) embedded molecularly imprinted membrane (QDs@MIM) was developed. The QDs@MIM was used as fluorescence artificial receptor for specific recognition and detection of target proteins. The QD@MIM was fabricated on a silylanized glass plate. Lysozyme, acrylamide, L-cysteine modified Mn²⁺-doped ZnS QDs and N,N'-methylenediacrylamide were used as template, functional monomer, assistant monomer and cross-linker, respectively. Under optimal conditions, the linear range for lysozyme ranged from 0.1 to 1.0 μmol/L, adsorption time was 4 min and the imprinting factor for lysozyme was 6.2. The results showed that the molecularly imprinted polymer can be used as a simple, rapid and selective biosensor to detect target proteins in biologic samples.
- molecularly imprinted membranes (MIM),
- quantum dots (QDs),
- protein detection
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