Citation: LI Wenjing, ZHAO Lijuan, WEI Chanling, DAI Xiaojun, GONG Bolin. Preparation and evaluation of chromatographic properties of sulfobetaine zwitterionic hydrophilic interaction chromatography stationary phase[J]. Chinese Journal of Chromatography, ;2017, 35(4): 382-387. doi: 10.3724/SP.J.1123.2016.09035 shu

Preparation and evaluation of chromatographic properties of sulfobetaine zwitterionic hydrophilic interaction chromatography stationary phase

1.
School of Chemistry & Chemical Engineering, Ningxia University, Yinchuan 750021, China;
2.
School of Chemistry & Chemical Engineering, Beifang University of Nationalities, Yinchuan 750021, China

Received Date: 17 September 2016

Fund Project: National Natural Science Foundation of China (Nos. 31360077, 31271868)

A zwitterionic sulfobetaine functional monomer N,N-dimethyl-N-acryloyloxyethyl-N-ammonium propane sulfonate (DMAEAPS) was synthesized. Dimethyl amino ethyl acrylate (DMAEA) and 1,3-propyl sulfonic acid ester as raw materials were used to synthesize the functional monomer. The DMAEAPS functional monomer was characterized by ¹H-NMR and FT-IR. 2-Bromoisobutyryl bromide allyl silicone (Sil-Br) was used as the initiator, CuBr and 2,2-bipyridyl (Bpy) as the catalytic system, and the functional monomer DMAEAPS was grafted on the surface of Sil-Br by atom transfer radical polymerization (ATRP). Then the sulfobetaine zwitterionic stationary phase (Sil-DMAEAPS) was obtained. The Sil-DMAEAPS stationary phase was characterized by element analysis. Chromatographic evaluation in hydrophilic interaction chromatography (HILIC) mode showed that the materials were suitable for use as stationary phase, and the column showed a dual retention mechanism, HILIC mode in acetonitrile-rich mobile phases and RP mode in highly aqueous mobile phases. The separation selectivity and retention of the five polar solutes (benzoin, pyridoxine, rutin hydrate, p-coumaric acid and caffeic acid) on the Sil-DMAEAPS stationary phase were different from the commercially available ZIC^®-HILIC. A method for the determination of rutin hydrate in rutin tablets was established. It is simple and accurate for the determination of polar compounds.
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沈阳化工大学材料科学与工程学院沈阳 110142