Citation: XU Jingjing, LIU Xing, ZHOU Hu. Recent advances in separation methods for post-translational modification proteomics[J]. Chinese Journal of Chromatography, ;2016, 34(12): 1199-1206. doi: 10.3724/SP.J.1123.2016.08047 shu

Recent advances in separation methods for post-translational modification proteomics

XU Jingjing ^1,2, ,
LIU Xing ¹ ,
ZHOU Hu ¹

1.
Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
2.
Chemical Drugs Department, Shanghai Institute of Food and Drug Control, Shanghai 201203, China

Received Date: 31 August 2016

Fund Project: National Natural Science Foundation of China (No. 21375138)

Post-translational modifications (PTMs) are the key control of many cellular processes. Current progresses of separation methods for PTMs proteomics, including reversed phase (RP) chromatography, ion exchange (IEX) chromatography, hydrophilic interaction chromatography (HILIC), porous graphitic carbon (PGC) chromatography, capillary electrophoresis (CE) and size-exclusion chromatography (SEC) are reviewed. The methods for the identification of PTMs have higher resolution and sensitivity. In addition, some other important separation methods for proteomics study are introduced, and they may be applied in the study of PTMs with further optimization in the future.
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