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Citation: GUO Zhenpeng, WANG Xiaoyu, CHEN Yi. Rapid determination of micro gibberellins by non-aqueous capillary electrophoresis with reversed electroosmotic flow[J]. Chinese Journal of Chromatography, ;2017, 35(1): 65-69. doi: 10.3724/SP.J.1123.2016.08042 shu

Rapid determination of micro gibberellins by non-aqueous capillary electrophoresis with reversed electroosmotic flow

GUO Zhenpeng ^1, ,
WANG Xiaoyu ³ ,
CHEN Yi ^1,2

1.
CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
2.
Beijing National Laboratory of Molecular Science, Beijing 100190, China;
3.
Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou 450001, China

Received Date: 31 August 2016

Fund Project: National Natural Science Foundation of China (Nos. 21235007, 21475136, 91117010).

A non-aqueous capillary electrophoresis (NACE) method for the rapid determination of micro gibberellins (GAs) was established. Dynamically poly(ethylene oxide) coated capillary was used, and electroosmotic flow (EOF) was reversed by using positive ions and adjusted by regulation the types and concentrations of the positive ions, running buffer, and pH of the buffer. With a buffer of 95% (v/v) methanol containing 10 mmol/L ammonium acetate at an acidity of 0.08% (v/v) acetic acid, the EOF was successfully reversed to separate the eight GAs in less than 10 min. Its applicability was validated by the determination of GAs in germinating wheat seeds, with relative standard deviations (RSDs)≤2.1% (intra-day) or ≤4.3% (inter-day) for migration times, and RSDs≤4.5% (intra-day) or≤6.9% (inter-day) for peak areas. The limits of detection (S/N=3) were in the range of 1.10-2.20 mg/L, with correlation coefficients (r²) of 0.9982-0.9993. The recoveries of the spiked samples were between 87.2% and 93.5%. The established method is simple, rapid, stable, and compatible with mass spectrometry, so it is valuable to be further studied.
- non-aqueous capillary electrophoresis (NACE),
- electroosmotic flow (EOF),
- dynamic coating,
- gibberellins (GAs)
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