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Citation: SUN Kaibo, SHANG Zhi, SUN Yan, QIAO Zhi, LIU Sha, Fan Liuyin, CAO Chengxi, XIAO Hua. Lectin based salivary glycoprotein separation, analysis and its application[J]. Chinese Journal of Chromatography, ;2016, 34(12): 1234-1239. doi: 10.3724/SP.J.1123.2016.08038 shu

Lectin based salivary glycoprotein separation, analysis and its application

State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

Received Date: 30 August 2016

Fund Project: National Natural Science Foundation of China (Nos. 21275099, 21305087, 21475086)

The abundance of glycoproteins in saliva is relatively low, which brings great challenge to their separation and analysis. In the study, wheat germ agglutinin (WGA) and aleuria aurantia lectin (AAL) were utilized to extract salivary glycoproteins. The effects of high abundant protein removal and two digestion methods on glycoprotein analysis were investigated. Our data demonstrated that the number of identified glycoproteins using WGA and AAL with in-gel digestion was significantly higher than that of in-solution digestion, as well as high abundant protein removal method. The developed strategy was further applied to compare the salivary glycoproteins from lung cancer patients and normal subjects. According to label free quantification results, 139 proteins were found and 102 proteins with the glycosylation sites were predicted. Among them, 14 glycoproteins showed significant difference between the cancer and control ones (p<0.05). Our study demonstrated that the developed strategy could be applied to the efficient separation and analysis of salivary glycoproteins and would contribute to the cancer biomarker discovery.
- lectin,
- glycoprotein,
- saliva,
- biomarker
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