Citation: ZHAI Guijin, WU Kui, WANG Fuyi. Separation, enrichment, mass spectrometric quantification and evaluation of serum phosphopeptides as potential tumor biomarkers[J]. Chinese Journal of Chromatography, ;2016, 34(12): 1192-1198. doi: 10.3724/SP.J.1123.2016.08037 shu

Separation, enrichment, mass spectrometric quantification and evaluation of serum phosphopeptides as potential tumor biomarkers

ZHAI Guijin ^1,2, ,
WU Kui ¹ ,
WANG Fuyi ^1,3

1.
CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
2.
Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, China;
3.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 30 August 2016

Fund Project: National Natural Science Foundation of China (Nos. 21135006, 21127901, 21275148, 21575145, 21321003)

Serum phosphopeptides has been a vital objective for the discovery of tumor biomarkers as their levels can reflect the changes in the activities of both endo-and exo-proteases, and the level of protein phosphorylation. Comprehensive analysis of serum phosphopeptides may lead to discovery of novel tumor biomarkers for clinical diagnosis. However, the extremely low abundance of serum phosphopeptides as well as high background level of highly abundant non-phosphoproteins/peptides makes the mass spectrometry (MS)-based phosphopeptide profiling a great challenge. In this review, we will summarize and review update advances in the separation, enrichment and MS quantification of phosphopeptides as well as the evaluation of serum phosphopeptides as potential tumor biomarkers. We will also prospect the future tendency and applications of this multi-disciplinary research field.
- phosphopeptides,
- separation and enrichment,
- mass spectrometric quantification,
- tumor biomarkers,
- serum,
- review
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