Citation: WENG Yejing, SUI Zhigang, ZHANG Lihua, ZHANG Yukui. Recent advances on exosomes isolation methods and proteomic analysis[J]. Chinese Journal of Chromatography, ;2016, 34(12): 1131-1136. doi: 10.3724/SP.J.1123.2016.08036 shu

Recent advances on exosomes isolation methods and proteomic analysis

1.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 30 August 2016

Fund Project: National Natural Science Foundation of China (No. 21305140)

Exosomes are membrane-bound vesicles secreted by cells with the diameter of 30-100 nm and the density of 1.10-1.18 kg/L. Exosomes exist widely in almost all kinds of biofluids and contain a series of biologically important molecules, such as lipids, proteins, mRNAs, miRNAs and ncRNAs. Emerging evidence shows that exosomes could establish an intercellular information transfer system, thereby affecting the physiological status of cells and involving in various pathological processes. Herein, the recent advances on exosome isolation and purification methods are reviewed. In the meantime, the proteomic analysis of exosomes is briefly introduced. Finally, the future of exosome proteomic research is discussed.
- exosomes,
- isolation and purification,
- proteomic,
- quantitative analysis,
- review,
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沈阳化工大学材料科学与工程学院沈阳 110142