Citation: ZHAO Tangjuan, CHEN Juan, SHI Yanping. Holistic analysis of Liuwei Dihuang pills using ultrasonic cell grinder extraction and ultra-performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2017, 35(1): 32-46. doi: 10.3724/SP.J.1123.2016.08025 shu

Holistic analysis of Liuwei Dihuang pills using ultrasonic cell grinder extraction and ultra-performance liquid chromatography

1.
Key Laboratory of Chemistry of Northwestern Plant Resources of Chinese Academy of Sciences and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 23 August 2016

Fund Project: National Natural Science Foundation of China (Nos. 21375136, 21575150) Scholar Program of West Light Project, Chinese Academy of Sciences.

An efficient holistic analysis strategy was developed and validated for quality control of the traditional Chinese medicine (TCM) preparation, Liuwei Dihuang pills (LWDHPs). In this strategy, eight bioactive components, i. e. allantoin, morroniside, loganin, peoniflorin, acteoside, paeonol, alisol B 23-acetate and pachymic acid from the six medicinal herbs composing LWDHPs were selected as the evaluation markers, and then they were simultaneously extracted by an ultrasonic cell grinder extraction (UCGE) method and separated in a single run by an ultra-performance liquid chromatographic method coupled with photodiode array detection (UPLC-PDA). Response surface methodology (RSM), a multivariate experimental design technique, was used to optimize the UCGE parameters based on Box-Behnken design (BBD) model. The optimum extraction conditions were obtained at a ratio of solvent to solid 45 mL/g, extraction time of 40 min and extraction temperature of 20℃. Under such conditions, the higher yields of the eight analytes were acquired compared with the traditional extraction methods, indicating that UCGE was a fast, easy and efficient method for extracting bioactive constituents from LWDHPs. Chromatographic separation was achieved on an HSS T3 column (50 mm×2.1 mm, 1.8 μm) using a linear gradient elution of acetonitrile and water. Because of the different UV characteristics of these components, five detection wavelengths were used for quantitative analysis. The proposed method was validated through linearity, limits of detection, limits of quantification, precision, stability, repeatability, and accuracy. The validated method was applied to analyze LWDHPs, which provided a reference for the quality evaluation of LWDHPs.
- ultrasonic cell grinder extraction (UCGE),
- response surface methodology (RSM),
- Liuwei Dihuang pills (LWDHPs),
- quality control
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沈阳化工大学材料科学与工程学院沈阳 110142