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Citation: LUO Dan, FENG Yuqi, YAO Jinting, SUN Youbao, HUANG Taohong, HASHI Yuki. Determination of oleanolic acid and ursolic acid in loquat leaf extract by chemical derivatization coupled with liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2017, 35(1): 27-31. doi: 10.3724/SP.J.1123.2016.08015 shu

Determination of oleanolic acid and ursolic acid in loquat leaf extract by chemical derivatization coupled with liquid chromatography-tandem mass spectrometry

LUO Dan ^1,2, ,
FENG Yuqi ¹ ,
YAO Jinting ² ,
SUN Youbao ² ,
HUANG Taohong ² ,
HASHI Yuki ²

1.
Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China;
2.
Shimadzu(China) Co., Ltd., Shanghai 200052, China

Received Date: 16 August 2016

Fund Project: National Natural Science Foundation of China (No. 21475098).

A method for the determination of oleanolic acid (OA) and ursolic acid (UA) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. By using derivatization reagents, N,N-dimethylethylenediamine (DMED) and d4-N,N-dimethylethylenediamine (d4-DMED), OA and UA in real samples and standard samples were derivatized separately. The derivative products of the standard samples were used as stable isotope internal standards, and accurately added to the samples and standard working solutions. Good linearities were obtained in the concentration range of 0.01-10 μ g/L with correlation coefficients (r²) greater than 0.99. The limits of detection of OA and UA were 0.92 ng/L and 1.06 ng/L, and the recoveries were between 98.7%-102.7% and 97.2%-105.0%, respectively. The method can be used for the detection of OA and UA in loquat leaf extract with high sensitivity.
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