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Citation: LI Yao, CHEN Jingya, WU Yulan, HAN Shuying, YU Hongli, WU Hao, CHI Yumei. Determination of the kinetics of photo-isomerization and equilibrium constant of resveratrol by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2016, 34(8): 783-787. doi: 10.3724/SP.J.1123.2016.03008 shu

Determination of the kinetics of photo-isomerization and equilibrium constant of resveratrol by high performance liquid chromatography

  • 1.

    Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China

  • Corresponding author: WU Hao,  CHI Yumei, 
  • Received Date: 6 March 2016

    Fund Project:

  • Trans-resveratrol (C14H12O3, 5-(4-hydroxystyryl)benzene-1, 3-diol), with a variety of physiological activities and abundant sources, shows instability to light irradiation. The control solution was balanced by photo-isomerization and high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of trans-resveratrol and cis-resveratrol. The methodology study showed good linear correlations (R2=0.999) in the range of 1.5-60 μ mol/L of trans-resveratrol and 1.5-55 μ mol/L of cis-resveratrol which conforms to the request of content determination. The method was used to study the kinetics and equilibrium constant of photo-isomerization. The results showed that trans-resveratrol protected from light was stable in neutral, weak acid and ethanol media at room temperature. The photo-isomerization equilibrium constant was 11.7±0.3 at 25 ℃ in dilute ethanol solution. The method of making the standard curve by photo-isomerization equilibrium solution is simple, convenient and practicable. The stability of trans-resveratrol is related to the medium. When pH value is less than 7, the photo-isomerization is a first order reaction.
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    1. [1]

      [1] Li J, Xiong X Y, Zeng J G, et al. Modern Chinese Medicine, 2013, 15(2): 100 李洁, 熊兴耀, 曾建国, 等. 中国现代中药, 2013, 15(2): 100

    2. [2]

      [2] Shiori T, Kenta S, Maki I, et al. Brain Res Bull, 2016, 120(1): 117

    3. [3]

      [3] Pema R, Basma M A, Xavier L L, et al. J Nutr Biochem, 2016, 28(1): 155

    4. [4]

      [4] Ramalingam P, Ko Y T. J Pharmaceut Biomed Anal, 2016, 119: 71  

    5. [5]

      [5] Li Y S, Wen J. Medical Recapitulate, 2008, 14(3): 469 黎永胜, 文军. 医学综述, 2008, 14(3): 469

    6. [6]

      [6] Baur J A, Pearson K J, Price N L, et al. Nature, 2006, 444(7117): 337  

    7. [7]

      [7] Xiao Z B, Zhao Y N, Liu J L, et al. Journal of Instrumental Analysis, 2015, 34(10): 1119 肖卓炳, 赵艺楠, 刘建兰, 等. 分析测试学报, 2015, 34(10): 1119

    8. [8]

      [8] Silva C G, Monteiro J, Marques R R N, et al. Photochem Photobio Sci, 2013, 12(4): 638  

    9. [9]

      [9] Liu Y P, Wen D W, Chen Z, et al. Chinese Journal of Chromatography, 2004, 22(6): 583 刘宇平, 文大为, 陈政, 等. 2004, 22(6): 583

    10. [10]

      [10] Orallo F. Curr Med Chem, 2006, 13(1): 87  

  • 加载中
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