Citation: LUO Birong, WAN Xu, DENG Xingliang, YU Yuanyuan, XIE Zhenwei. Simultaneous determination of 18 phenol pollutants in waste water by high performance liquid chromatography- tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(5): 473-480. doi: 10.3724/SP.J.1123.2016.02018 shu

Simultaneous determination of 18 phenol pollutants in waste water by high performance liquid chromatography- tandem mass spectrometry

1.
Sichuan Environmental Monitoring Center, Chengdu 610091, China

Corresponding author: LUO Birong,
Received Date: 23 February 2016

Fund Project: 国家环境保护标准制定项目(2014-35). (2014-35)

A method was developed for the simultaneous determination of 18 phenol pollutants in waste water by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A volume of 5.0 mL waste water was placed into a centrifuge tube. Ammonia was added to the waste water to adjust pH≥12. A volume of 1.0 mL methylene chloride-hexane solution (2: 1, v/v) was added to the waste water, then the centrifuge tube was oscillated for 5 min and centrifuged for 5 min at 4000 r/min. The water sample was filtered through a 0.22 μ m polytetrafluoroethylene (PTFE) microfiltration membrane and adjusted to neutral by formic acid. With methanol-0.01 mol/L ammonium formate/formic acid aqueous solution (pH 4.0) as mobile phase, the separation was performed on a Thermo Hypersil ODS column (100 mm×2.1 mm, 5.0 μ m) in gradient elution. The flow rate was 0.2 mL/min. The column temperature was 30℃. The samples were detected by multiple reaction monitoring (MRM) mode with negative electrospray ionization. The phenol pollutants were quantified by external standard method. The calibration curves of the phenol pollutants showed good linearities in a suitable range with correlation coefficients (r²) not less than 0.9991. The detection limits of phenol pollutants ranged from 0.10 μ g/L to 0.88 μ g/L. The relative standard deviations of phenol pollutants were 2.5%-9.9% (n=6). The average recoveries of the 18 phenol pollutants spiked in waste water samples ranged from 68.7% to 118%(n=3). The method has been proven to be sensitive, rapid and little interference. It is suitable for the determination of the 18 phenol pollutants simultaneously in environmental waste water.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- phenol pollutants,
- waste water
1. [1]
  [1] Toxic Chemicals Management Office of State Environmental Protection Agency, Institute of Environmental Protection, Beijing Chemical Industry Research Institute, Ministry of Chemical Industry, ed. Chemical Toxicity, Statute and Environmental Data Handbook. Beijing: China Environmental Science Press, 1992 国家环境保护局有毒化学品管理办公室、化工部北京化工研究院环境保护研究所, 编. 化学品毒性、法规、环境数据手册. 北京: 中国环境科学出版社, 1992
2. [2]
  [2] Zhou W M, Fu D Q, Sun Z G. Research of Environmental Sciences, 1991, 4(6): 9 周文敏, 傅德黔, 孙宗光. 环境科学研究, 1991, 4(6): 9
3. [3]
  [3] Kou L J, Liang R N. Chinese Journal of Chromatography, 2014, 32(8): 817 寇立娟, 梁荣宁. 色谱, 2014, 32(8): 817
4. [4]
  [4] Ding J, Zhang S H, Liu J N, et al. Chinese Journal of Chromatography, 2014, 32(5): 529 丁洁, 张圣虎, 刘济宁, 等. 色谱, 2014, 32(5): 529
5. [5]
  [5] Liu Y, Zhang T L, Yang L, et al. Acta Armamentarii, 2015, 36(1): 70 刘玉, 张同来, 杨利, 等. 兵工学报, 2015, 36(1): 70
6. [6]
  [6] Xu D D, Tian Z. Guangdong Chemical Industry, 2014, 41(17): 160 徐丹丹, 田震. 广东化工, 2014, 41(17): 160
7. [7]
  [7] Fang Z Q, Li Q H, He H Z, et al. Journal of Sichuan Normal University: Natural Science, 2013, 36(1): 107 方志青, 李秋华, 贺华中, 等. 四川师范大学学报: 自然科学版, 2013, 36(1): 107
8. [8]
  [8] Noestheden M, Noot D, Hindle R. J Chromatogr A, 2012, 1263: 68
9. [9]
  [9] Wang X Y, Qi W M, Zhao X E, et al. Chinese Journal of Chromatography, 2014, 32(6): 623 王晓燕, 亓伟梅, 赵先恩, 等. 色谱, 2014, 32(6): 623
10. [10]
  [10] Gong S X, Wang X, Chen Y, et al. J Chromatogr A, 2015, 1401: 17
11. [11]
  [11] Wang L, Zhu H, Sun Y T, et al. Chinese Journal of Analytical Chemistry, 2011, 39(5): 709 王良, 朱红, 孙艳涛, 等. 分析化学, 2011, 39(5): 709
12. [12]
  [12] Gou X L, Gao X, Hu G H, et al. Chinese Journal of Chromatography, 2014, 32(9): 988 勾新磊, 高峡, 胡光辉, 等. 色谱, 2014, 32(9): 988
13. [13]
  [13] Li S H, Zhao S P, Li Y F. Henan Science, 2014, 32(2): 169 李淑红, 赵仕沛, 李迎芳. 河南科学, 2014, 32(2): 169
14. [14]
  [14] Li Z C, Tang W R, Zhu J K, et al. Journal of East China Normal University: Natural Science, 2013(1): 128 李自成, 唐菀融, 朱金坤, 等. 华东师范大学学报: 自然科学版, 2013(1): 128
15. [15]
  [15] GB 21523-2008
16. [16]
  [16] GB 8978-1996
17. [17]
  [17] GB 18918-2002
18. [18]
  [18] GB 14470.2-2002
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Changjun You , Chunchun Wang , Mingjie Cai , Yanping Liu , Baikang Zhu , Shijie Li . Improved Photo-Carrier Transfer by an Internal Electric Field in BiOBr/N-rich C₃N₅ 3D/2D S-Scheme Heterojunction for Efficiently Photocatalytic Micropollutant Removal. Acta Physico-Chimica Sinica, 2024, 40(11): 2407014-0. doi: 10.3866/PKU.WHXB202407014
3. [3]
  Ling WANG , Weipeng YAN , Zhuoyi ZHENG , Sihan ZHU , Mingxian GONG , Xiangyu MA . Fabrication of biochar-supported nano zero-valent iron and its high-efficiency performance for Cr(Ⅵ) removal from wastewater. Chinese Journal of Inorganic Chemistry, 2025, 41(12): 2441-2454. doi: 10.11862/CJIC.20250264
4. [4]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
5. [5]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
6. [6]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
7. [7]
  Jing Wang , Pingping Li , Yuehui Wang , Yifan Xiu , Bingqian Zhang , Shuwen Wang , Hongtao Gao . Treatment and Discharge Evaluation of Phosphorus-Containing Wastewater. University Chemistry, 2024, 39(5): 52-62. doi: 10.3866/PKU.DXHX202309097
8. [8]
  Hongbo Zhang , Yihong Tang , Suxia Zhang , Yuanting Li . Electrochemical Monitoring of Photocatalytic Degradation of Phenol Pollutants: A Recommended Comprehensive Analytical Chemistry Experiment. University Chemistry, 2024, 39(6): 326-333. doi: 10.3866/PKU.DXHX202310013
9. [9]
  Xinxin YU , Yongxing LIU , Xiaohong YI , Miao CHANG , Fei WANG , Peng WANG , Chongchen WANG . Photocatalytic peroxydisulfate activation for degrading organic pollutants over the zero-valent iron recovered from subway tunnels. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 864-876. doi: 10.11862/CJIC.20240438
10. [10]
  Zhiqiang XING , Jinling LIU , Mingmin SU , Lei ZHANG , Lijun YANG . CoNi dual-single-atom catalyst for electrocatalytic H₂O₂ production and in situ electro-Fenton degradation of pollutants. Chinese Journal of Inorganic Chemistry, 2025, 41(12): 2479-2490. doi: 10.11862/CJIC.20250181
11. [11]
  Yuanqing Wang , Yusong Pan , Hongwu Zhu , Yanlei Xiang , Rong Han , Run Huang , Chao Du , Chengling Pan . Enhanced Catalytic Activity of Bi₂WO₆ for Organic Pollutants Degradation under the Synergism between Advanced Oxidative Processes and Visible Light Irradiation. Acta Physico-Chimica Sinica, 2024, 40(4): 2304050-0. doi: 10.3866/PKU.WHXB202304050
12. [12]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
13. [13]
  Ping Ye , Lingshuang Qin , Mengyao He , Fangfang Wu , Zengye Chen , Mingxing Liang , Libo Deng . Potential of Zero Charge-Mediated Electrochemical Capture of Cadmium Ions from Wastewater by Lotus Leaf-Derived Porous Carbons. Acta Physico-Chimica Sinica, 2025, 41(3): 100023-0. doi: 10.3866/PKU.WHXB202311032
14. [14]
  Chunai Dai , Yongsheng Han , Luting Yan , Zhen Li , Yingze Cao . Preparation of Superhydrophobic Surfaces and Their Application in Oily Wastewater Treatment: Design of a Comprehensive Physical Chemistry Innovation Experiment. University Chemistry, 2024, 39(2): 34-40. doi: 10.3866/PKU.DXHX202307081
15. [15]
  Qianqian Zhong , Yucui Hao , Guotao Yu , Lijuan Zhao , Jingfu Wang , Jian Liu , Xiaohua Ren . Comprehensive Experimental Design for the Preparation of the Magnetic Adsorbent Based on Enteromorpha Prolifera and Its Utilization in the Purification of Heavy Metal Ions Wastewater. University Chemistry, 2024, 39(8): 184-190. doi: 10.3866/PKU.DXHX202312013
16. [16]
  Simin Fang , Wei Huang , Guanghua Yu , Cong Wei , Mingli Gao , Guangshui Li , Hongjun Tian , Wan Li . Integrating Science and Education in a Comprehensive Chemistry Design Experiment: The Preparation of Copper(I) Oxide Nanoparticles and Its Application in Dye Water Remediation. University Chemistry, 2024, 39(8): 282-289. doi: 10.3866/PKU.DXHX202401023
17. [17]
  Shuhong Xiang , Lv Yang , Yingsheng Xu , Guoxin Cao , Hongjian Zhou . Selective electrosorption of Cs(Ⅰ) from high-salinity radioactive wastewater using CNT-interspersed potassium zinc ferrocyanide electrodes. Acta Physico-Chimica Sinica, 2025, 41(9): 100097-0. doi: 10.1016/j.actphy.2025.100097
18. [18]
  Zhongyan Cao , Shengnan Jin , Yuxia Wang , Yiyi Chen , Xianqiang Kong , Yuanqing Xu . Advances in Highly Selective Reactions Involving Phenol Derivatives as Aryl Radical Precursors. University Chemistry, 2025, 40(4): 245-252. doi: 10.12461/PKU.DXHX202405186
19. [19]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
20. [20]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(533)
HTML views(32)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142