Citation: WANG Yongzhong, LIU Qing, JIANG Hui, HAN Yanquan, LI Yuxin. Metabolomics study in rat urine of adjuvant arthritis using gas chromatography-time-of-flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(6): 602-607. doi: 10.3724/SP.J.1123.2016.01020 shu

Metabolomics study in rat urine of adjuvant arthritis using gas chromatography-time-of-flight mass spectrometry

WANG Yongzhong ^1,*,, ,
LIU Qing ² ,
JIANG Hui ¹ ,
HAN Yanquan ¹ ,
LI Yuxin ²

1.
The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of Traditional Chinese Medicine(TCM), Hefei 230031, China
2.
College of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230038, China

Corresponding author: WANG Yongzhong, wyzhmail@163.com
Received Date: 15 January 2016

Fund Project: Project of Natural Science Foundation of Anhui Province No. 1508085MH201

Adjuvant arthritis (AA) rats induced by Freund's complete adjuvant (FCA) were used to observe the changes of the degree of swelling in the toes and pathomorphology of ankle joint tissue. Gas chromatography-time-of-flight mass spectrometry (GC-TOF MS) was employed to detect the metabolite spectrum in rat urine with AA, and the data were analyzed by principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), and to explore its possible pathogenesis. Several metabolites based on the variable importance in the projection values (VIP>1), P value (<0.05) and screening in databases were identified. The results showed that 20 potential biomarkers such as isocitric acid, α-ketoglutaric acid, citraconic acid, creatine and 3-hydroxybutyric acid which had a significant contribution to classification were selected. Comparing with the normal group, AA rats present an abnormal metabolism. This study illustrates that AA metabolomics pathogenesis may be related to regulating the dysfunction of energy metabolism, amino acid metabolism and fatty acid metabolism.
- gas chromatography-time-of-flight mass spectrometry (GC-TOF MS),
- adjuvant arthritis (AA),
- metabolomics,
- urine
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