Citation: WENG Zhongya, XUE Yun, SHI Wenjun, WANG Yan, YAN Chao. Preparation of a new organic-silica hybrid monolithic column and its applications in capillary liquid chromatography and pressurized capillary electrochromatography[J]. Chinese Journal of Chromatography, ;2016, 34(5): 467-472. doi: 10.3724/SP.J.1123.2015.12040 shu

Preparation of a new organic-silica hybrid monolithic column and its applications in capillary liquid chromatography and pressurized capillary electrochromatography

1.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: WANG Yan, YAN Chao,
Received Date: 1 January 2016

Fund Project: 国家自然科学基金项目(21175092) (21175092)上海市科委科研计划项目(15142200200) (15142200200)上海市教育委员会科研创新项目(14YZ170) (14YZ170)中国博士后科学基金项目(2015M581628). (2015M581628)

The organic-silica hybrid poly(POSS-co-AM) monolithic columns were prepared by in-situ polymerization process with octavinyl-T8-silsesquioxane (POSS) as a crosslinking agent and acrylamide (AM) as monomer, tetrahydrofuran (THF) as a porogenic agent and azobisisobutyronitrile (AIBN) as initiator. In order to optimize the properties, the composition of the polymerization mixture was investigated. The optimum preparation conditions were as follows: both the mass ratios of monomer to porogen and POSS to AM were 1.0: 5.0; the mass fraction of the initiator (AIBN) was 0.1%. With the help of POSS materials, homogeneous column bed and good permeability were obtained. The columns were used in capillary liquid chromatography (cLC) and pressurized capillary electrochromatography (pCEC) for the separation of mixtures of amines, nucleosides, nitrobenzene amines. Satisfactory separations for these samples are achieved. The column is proved to be useful in both reversed-phase chromatography (RPLC) and hydrophilic interaction chromatography (HILIC).
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沈阳化工大学材料科学与工程学院沈阳 110142