Citation: SHI Wenjun, TIAN Huajun, XUE Yun, WENG Zhongya, QU Qishu, WANG Yan, YAN Chao. Performance evaluation of 1.2 μ m fibrous core-shell packing material for pressurized capillary electrochromatography[J]. Chinese Journal of Chromatography, ;2016, 34(5): 461-466. doi: 10.3724/SP.J.1123.2015.12039 shu

Performance evaluation of 1.2 μ m fibrous core-shell packing material for pressurized capillary electrochromatography

SHI Wenjun ¹ ,
TIAN Huajun ¹ ,
XUE Yun ¹ ,
WENG Zhongya ¹ ,
QU Qishu ² ,
WANG Yan ^1,, ,
YAN Chao ^1,,

1.
1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China;
2.
2. School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China

Corresponding author: WANG Yan, YAN Chao,
Received Date: 31 December 2015

Fund Project: 国家自然科学基金项目(21175092) (21175092)上海市科委科研计划项目(15142200200) (15142200200)上海市教育委员会科研创新项目(14YZ170) (14YZ170)中国博士后科学基金项目(2015M581628). (2015M581628)

Silica microsphere packing material with fibrous shells (1.2 μ m) was successfully synthesized and bonded with octadecylsilane functionality. These stationary phase particles were packed into fused-silica capillary with 100 μ m i.d. for a total length of 350 mm (70 mm effective length), which was evaluated for pressurized capillary electrochromatography (pCEC). The efficiency of the C18 reversed-phase column was characterized through the theoretical plates of thiourea and naphthalene. The effects of experimental parameters such as the content of acetonitrile in the mobile phase, the concentration of the buffer solution, the pH value of the mobile phase, the flow rate, the applied voltage and the on-column efficiency were investigated. The results showed a typical reversed-phase chromatographic performance. The eight neutral compounds were baseline separated within 8 min and the column efficiency as high as 190792 plate/m for benzophenone was obtained with the optimal conditions of 10 mmol/L phosphate buffer (pH 7.2) in 70% (v/v) acetonitrile aqueous solution at an applied negative voltage of 10 kV and a supplementary pressure of 1.66×10⁷ Pa. The optimal linear velocity was 1 mm/s. The research work confirmed the feasibility of using 1.2 μ m silica microsphere packing material with fibrous shells as a novel stationary phase for pCEC.
1. [1]
  [1] Horvath C G, Preiss B A, Lipsky S R. Anal Chem, 1967, 39(12): 1422
2. [2]
  [2] Knox J H, Chem A. Anal Chem, 1966, 38(2): 253
3. [3]
  [3] Kirkland J J, Chem A. Anal Chem, 1969, 41(1): 218
4. [4]
  [4] Kirkland J J, Langlois T J. US Patent, 20070189944. 2007-08-06
5. [5]
  [5] Dong H J, Brennan J D. Chem Commun, 2011, 47(4): 1207
6. [6]
  [6] Büchel G, Unger K K, Matsumoto A, et al. Adv Mater, 1998, 10(13): 1036
7. [7]
  [7] Dong H J, Brennan J D. J Mater Chem, 2012, 22(26): 13197
8. [8]
  [8] Yoon S B, Kim J Y, Kim J H, et al. J Mater Chem, 2007, 17(18): 1758
9. [9]
  [9] Yoo W C, Stein A. Chem Mater, 2011, 23(7): 1761
10. [10]
  [10] Chen W, Wei T C. EP Patent, 2218500A2, 2010-08-18
11. [11]
  [11] Kirkland J J, Truszkowski F A, Dilks C H, et al. J Chromatogr A, 2000, 890: 3
12. [12]
  [12] Honda H, Kimura M, Honda F, et al. Colloid Surface A, 1994, 82(2): 117
13. [13]
  [13] Polshettiwar V, Cha D, Zhang X X, et al. Angew Chem Int Edit, 2010, 49(50): 9652
14. [14]
  [14] Min Y, Jiang B, Wu C, et al. J Chromatogr A, 2014, 1356: 148
15. [15]
  [15] Qu Q S, Min Y, Zhang L H, et al. Anal Chem, 2015, 87(19): 9631
16. [16]
  [16] Jiang Z J, Gao R Y, Zhou Y B, et al. J Microcolumn Sep, 2001, 13(5): 191
17. [17]
  [17] Yan C. US Patent, 6569325 B1. 2003-05-27
18. [18]
  [18] Ma E J, Xue Y Y, Wang Y, et al. Chinese Journal of Antibiotics, 2011, 36(7): 536 玛尔江, 薛云云, 王彦, 等. 中国抗生素杂志, 2011, 36(7): 536
19. [19]
  [19] Jin H, Fan S H, Wang Y, et al. Journal of Instrumental Analysis, 2010, 29(5): 519 金慧, 范松华, 王彦, 等. 分析测试学报, 2010, 29(5): 519
20. [20]
  [20] Wang X X, Wang Y, Li J, et al. Science and Technology of Food Industry, 2015(9): 273 王晓曦, 王彦, 李静, 等. 食品工业科技, 2015(9): 273
21. [21]
  [21] Zhang H P, Wang Y, Gu X, et al. Electrophoresis, 2011, 32(3/4): 340
22. [22]
  [22] Lu Y F, Wang H, Wang G M, et al. Chinese Journal of Chromatography, 2015, 33(3): 209 鲁阳芳, 汪慧, 王桂明, 等. 色谱, 2015, 33(3): 209
23. [23]
  [23] Lu Y F, Wang H, Xue Y, et al. Electrophoresis, 2015, 36(17): 2120
24. [24]
  [24] Zhou W L, Kan W B, Wang Y H, et al. Journal of Instrumental Analysis, 2015, 34(3): 321 周文莉, 阚文彬, 王玉红, 等. 分析测试学报, 2015, 34(3): 321
25. [25]
  [25] Wan Q Y, Ru X, Wang X X, et al. Chinese Journal of Analytical Chemistry, 2015, 43(7): 1063 万青云, 茹鑫, 王晓曦, 等. 分析化学, 2015, 43(7): 1063
26. [26]
  [26] Shan M, Li J, Ru X, et al. Journal of Analytical Science, 2015, 31(4): 445 山梅, 李静, 茹鑫, 等. 分析科学学报, 2015, 31(4): 445
27. [27]
  [27] [28]
  [28] Wu Q, Yu X W, Wang Y, et al. Electrophoresis, 2014, 35(17): 2470
28. [29]
  [29] Zheng S, Shi W J, Wang X X, et al. Food Science, 2014, 20(20): 105 郑署, 施文君, 王晓曦, 等. 食品科学, 2014, 20(20): 105
29. [30]
  [30] Yan R H, Gao Y, Wang X C, et al. J Chromatogr Sci, 2013, 51: 468
30. [31]
  [31] Wang H, Xue Y, Lu Y F, et al. Chinese Journal of Chromatography, 2015, 33(3): 215 汪慧, 薛芸, 鲁阳芳, 等. 色谱, 2015, 33(3): 215
31. [32]
  [32] Rocchi S, Fanali S, Farkas T, et al. J Chromatogr A, 2014, 1363: 363
32. [33]
  [33] Fanali S, D'Orazio G, Farkas T, et al. J Chromatogr A, 2012, 1269: 136
33. [34]
  [34] Zhang B, Bergström E T, Goodall D M, et al. Anal Chem, 2007, 79(23): 9229
34. [35]
  [35] Zhang X H, Wang Y, Gu X, et al. Chinese Journal of Analytical Chemistry, 2011, 39(4): 455 张晓辉, 王彦, 谷雪, 等. 分析化学, 2011, 39(4): 455
35. [36]
  [36] Berthod A, Chang C D, Armstrong D W. Talanta, 1993, 40(9): 1367
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