Citation: WU Haiyan, GUO Mengmeng, ZHAO Chunxia, TAN Zhijun, GU Haifeng, ZHAI Yuxiu, LU Lina. Liquid chromatography-tandem mass spectrometry analysis for azaspiracids and their metabolites[J]. Chinese Journal of Chromatography, ;2016, 34(4): 401-406. doi: 10.3724/SP.J.1123.2015.11043 shu

Liquid chromatography-tandem mass spectrometry analysis for azaspiracids and their metabolites

WU Haiyan ^1,2 ,
GUO Mengmeng ^1,2 ,
ZHAO Chunxia ^1,2 ,
TAN Zhijun ^1,2,, ,
GU Haifeng ³ ,
ZHAI Yuxiu ^1,2 ,
LU Lina ^1,2

1.
1. Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2.
2. Laboratory of Quality & Safety Risk Assessment for Aquatic Products(Qingdao), Ministry of Agriculture, Qingdao 266071, China;
3.
3. Third Institute of Oceanography, State Oceanic Administration, Xiamen 361000, China

Corresponding author: TAN Zhijun,
Received Date: 26 November 2015

Fund Project: 国家自然科学基金项目(41106109) (41106109)中央级基本科研业务费项目(20603022011004,20603022015017-2). (20603022011004,20603022015017-2)

A liquid chromatography-tandem mass spectrometry method was developed for the analysis of azaspiracids and their metabolites. Both target metabolites and non-objective compounds detection methods were investigated, and the secondary mass spectra were compared with the standard spectra libraries to achieve high precision qualitative analysis. As a result, 11 metabolites including AZA-2, 3, 6, 11, 12, 16, 17, 28 and 36, as well as the glutathione conjugated metabolites of AZAs were analyzed in toxin-producing algae, accumulated samples of metabolic experiments and positive samples. Conventional metabolites were unbiased detected by target metabolites detection method, and showed a better response to low concentration metabolites. The method has excellent reproducibility, and is easy for operators. It can be used for the routine monitoring of AZAs.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- azaspiracids (AZAs),
- metabolites,
- qualitative analysis
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