Citation: ZHANG Jing, CHEN Xiaodong, LI Liqun, HE Jianfeng, FAN Jun, ZHANG Weiguang. Comparative separation of chiral compounds by supercritical fluid chromatography and high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2016, 34(3): 321-326. doi: 10.3724/SP.J.1123.2015.11025 shu

Comparative separation of chiral compounds by supercritical fluid chromatography and high performance liquid chromatography

ZHANG Jing ¹ ,
CHEN Xiaodong ² ,
LI Liqun ¹ ,
HE Jianfeng ¹ ,
FAN Jun ^1,, ,
ZHANG Weiguang ^1,2,,

1.
1. School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China;
2.
2. Guangzhou Research & Creativity Biotechnology Co. Ltd., Guangzhou 510663, China

Corresponding author: FAN Jun, ZHANG Weiguang,
Received Date: 14 November 2015

Fund Project: 国家自然科学基金项目(21171059,21571070) (21171059,21571070)科技型中小企业技术创新基金项目(13C26214404534) (13C26214404534)广东省科技计划项目(2012B010900043,2014A010101145) (2012B010900043,2014A010101145)广州市科技计划项目(2013J4400027,201508020093). (2013J4400027,201508020093)

Supercritical fluid chromatographic (SFC) technique has been widely applied in the analysis, determinations and chromatographic preparations of chiral compounds in past decades due to some advantages such as fast speed, high efficiency and low consumption of organic solvents. Herein, 24 kinds of chiral compounds (H1-H24) have been resolved on four polysaccharide-coated chiral stationary phases (CSPs) by SFC and high performance liquid chromatography (HPLC), separately. Taking into account their retention times and selection factors of these compounds, SFC separation showed a better efficiency than HPLC for most of the compounds, except for some axis-chiral compounds. Some complementarity between SFC and HPLC separations was observed. For H15-H17, their retentions in SFC separations showed a strengthening trend with increasing carbon numbers of side alkyl chain, whereas a reverse tendency in HPLC separation was displayed. Most interestingly, an elution order reversal of metconazole enantiomers was found in this work. In brief, these results would provide some valuable references for the development of SFC separation methods in the future.
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