Citation: XU Xu, XIAO Yuancan, GENG Dandan, PI Li, DONG Qi, HU Fengzu. Simultaneous determination of 16 sulfonamide residues in beef by high performance liquid chromatography-fluorescence detection with online post-column derivatization[J]. Chinese Journal of Chromatography, ;2016, 34(4): 422-428. doi: 10.3724/SP.J.1123.2015.11020 shu

Simultaneous determination of 16 sulfonamide residues in beef by high performance liquid chromatography-fluorescence detection with online post-column derivatization

XU Xu ^1,2 ,
XIAO Yuancan ^1,, ,
GENG Dandan ^1,2 ,
PI Li ¹ ,
DONG Qi ¹ ,
HU Fengzu ¹

1.
1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;
2.
2. University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: XIAO Yuancan,
Received Date: 12 November 2015

Fund Project: 中国科学院仪器功能开发技术创新项目(Ig201308) (Ig201308)中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26) (KSCX2-EW-J-26)青海省科技计划项目(2015-ZL-Y23) (2015-ZL-Y23)青海省科技平台建设项目(2012-T-Y19). (2012-T-Y19)

In this study, we investigated the effects of the four kinds of derivatization reagents, including fluorescamine, o-phthaladehyde, fluorescein isothiocyanate isomer and 2, 3-naphthalenedicarboxaldehyde. A high performance liquid chromatography with fluorescence detection method for the rapid determination of 16 sulfonamide residues in beef was developed by using improved QuEChERS method for sample pretreatment and fluorescamine for online post-column derivatization. The beef samples were extracted with acetonitrile containing 1%(v/v) acetic acid, cleaned-up by QuEChERS method, injected for online post-column derivatization with fluorescamine, and then analysed with fluorescence detection. Under the optimized conditions of QuEChERS and chromatography, the 16 sulfonamide residues showed good linearities in the range of 0.024-2.533 mg/L with the correlation coefficients (r) higher than 0.992. The limits of detection of the method were 1.6 to 8.2 μg/kg. The average recoveries of the 16 sulfonamide residues were in the range of 66.6%-109.5% with the relative standard deviations from 0.9% to 9.9%. The method has the advantages of rapidity, simplicity, high sensitivity and better purification effect. It is suitable for the rapid determination of the sulfonamide residues in beef.
- QuEChERS,
- fluorescamine,
- online post-column derivatization,
- high performance liquid chromatography-fluorescence detection (HPLC-FLR),
- sulfonamides,
- beef
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