Citation: HUANG Fang, WU Huiqin, HUANG Xiaolan, LUO Huitai, ZHU Zhixin, LIN Xiaoshan, MA Liguo, JIANG Yalan. Simultaneous determination of 17 aphrodisiac chemical drugs illegally added in health products and Chinese patent medicines by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(3): 270-278. doi: 10.3724/SP.J.1123.2015.11016 shu

Simultaneous determination of 17 aphrodisiac chemical drugs illegally added in health products and Chinese patent medicines by high performance liquid chromatography-tandem mass spectrometry

1.
1. Guangdong Provincial Public Laboratory of Analysis and Testing Technology, China National Analytical Center (Guangzhou), Guangzhou 510070, China;
2.
2. College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;
3.
3. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510024, China

Received Date: 16 November 2015

Fund Project: 广东省促进科技服务业发展计划项目(2012B040302007). (2012B040302007)

A method was proposed for the simultaneous determination of seventeen aphrodisiac chemical drugs which were illegally mixed into the health products and Chinese patent medicines by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). The extraction, chromatographic conditions and mass spectrometry parameters were optimized in order to separate three groups of isomers. The drugs were extracted with methanol under ultrasonic condition, separated on an Agilent Extend C18 chromatographic column (100 mm×2.1 mm, 3.5 μm) with acetonitrile and water (additional 10 mmol/L ammonium acetate) as mobile phases under gradient elution at a flow rate of 0.25 mL/min. The determination was conducted by tandem mass spectrometry in positive ESI mode under multiple reaction monitoring (MRM) mode. The method made seventeen aphrodisiac chemical drugs as well as the three groups of isomers to get the ideal separation and accurate quantitative. The method was proved to be rapid, selective, sensitive and stable, and it has been applied to the screening and detection of aphrodisiac chemical drugs illegally added in health products and Chinese patent medicines. All the tested samples show that the aphrodisiac chemical drugs have a high detection rate in the recent three years. The relevant regulatory authorities should pay more attention to it.
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