Citation: LIU Wenye, QIAO Hong, ZHAO Shan, LI Jiang, DING Xiaojing. Determination of polyhexamethylene guanide, polyhexamethylene biguanide, chlorhexidine acetate and benzethonium chloride in compound chemical disinfectants by micellar electrokinetic chromatography with bi-micelle[J]. Chinese Journal of Chromatography, ;2016, 34(3): 332-339. doi: 10.3724/SP.J.1123.2015.10029 shu

Determination of polyhexamethylene guanide, polyhexamethylene biguanide, chlorhexidine acetate and benzethonium chloride in compound chemical disinfectants by micellar electrokinetic chromatography with bi-micelle

LIU Wenye ^1,2 ,
QIAO Hong ² ,
ZHAO Shan ² ,
LI Jiang ² ,
DING Xiaojing ^1,2,,

1.
1. College of Public Health, Capital Medical University, Beijing 100069, China;
2.
2. Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Diseases Control and Prevention, Beijing 100013, China

Corresponding author: DING Xiaojing,
Received Date: 19 October 2015

Fund Project: 北京市卫生系统高层次卫生技术人才培养项目(2013-2-036). (2013-2-036)

A new method for the accurate assay of four components (polyhexamethylene guanide (PHMG), polyhexamethylene biguanide (PHMB), chlorhexidine acetate (CHA) and benzethonium chloride (BTC)) in compound chemical disinfectants by micellar electrokinetic chromatography (MEKC) with bi-micelle was developed. An uncoated fused-silica capillary with 50 μm i.d. and 50.2 cm total length (effective length: 40 cm) under a separation voltage of 24 kV was used. The separation buffer consisted of 20 mmol/L Na₂B₄O₇, 30 mmol/L sodium dodecyl sulfate (SDS), 5 mmol/L sodium deoxycholate (SD) and 0.8 g/L polyethyleneglycol (PEG) 20000. A 1 : 10 dilution of the running buffer with ultrapure water was used as the sample buffer. The injection pressure and time were 3.448 kPa and 12 s, respectively. Liquid samples could be directly injected after dilution with sample buffer. Solid samples were extracted with 10 mL of sample buffer twice and the two extracted solutions were merged together and then directly injected. The factors such as the buffer concentration and pH, the concentrations of SDS and SD, the concentration of PEG 20000 and the sample extraction solution which influence the separation were investigated in detail. The corrected peak areas versus the concentrations of the four components showed good linear relationships within the range from 3 mg/L to 140 mg/L with correlation coefficients (r) greater than 0.999. The limits of detection (LODs) and the limits of quantification (LOQs) of the four components were all 1 mg/L and 3 mg/L, respectively. The average recoveries at three spiked levels were in the range of 84.1%-109.6% with RSDs all less than 6%. Eleven samples were analyzed and the results met well with the specified content. The PHMG could be easily discriminated from PHMB. The MEKC method is suitable for the supervision of compound chemical disinfectants.
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