Citation: YANG Simei, ZHANG Jiayao, LI Fei, HU Xufang, CAO Qiue. Separation and determination of optical isomers of phenylephrine by chiral ligand exchange capillary electrophoresis coupling with the promoting effect of ionic liquid[J]. Chinese Journal of Chromatography, ;2016, 34(1): 103-107. doi: 10.3724/SP.J.1123.2015.10013 shu

Separation and determination of optical isomers of phenylephrine by chiral ligand exchange capillary electrophoresis coupling with the promoting effect of ionic liquid

1.
Key Laboratory of Medicinal Chemistry for Nature Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China

Corresponding author: CAO Qiue,
Received Date: 12 October 2015

Fund Project: 国家自然科学基金项目(21465025). (21465025)

A method for the separation and determination of optical isomers of phenylephrine was developed based on the promoting effect of non-chiral ionic liquid on chiral ligand-exchange capillary electrophoresis after the electrophoretic parameters were optimized systematically. R-phenylephrine and S-phenylephrine can be separated and determined effectively in 20 mmol/L Tris-H₃PO₄ buffer solution (pH 5.4) composed of 4.0 mmol/L Cu(Ⅱ), 8.0 mmol/L L-proline (L-Pro) and 15 mmol/L 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) with the applied voltage of 20 kV, capillary temperature of 25 ℃, detection wavelength of 254 nm, and injection of 5 s at 3447 Pa. The resolution of R- and S-phenylephrines was 1.42. The linear ranges for the determination of R-phenylephrine and S-phenylephrine were 12.5-150 mg/L and 15.0-150 mg/L, respectively. The method has been satisfactorily used for the determination of R-phenylephrine and S-phenylephrine in the spiked blood and urine samples. The spiked recoveries in the urine sample were in the range of 93.7%-108.2% with the RSDs lower than 3.18%(n=3), and the spiked recoveries in the blood sample were in the range of 91.4% and 113.1% with the RSDs lower than 4.82%(n=3).
- capillary electrophoresis,
- chiral ligand exchange,
- ionic liquid,
- phenylephrine,
- optical isomers,
- chiral separation
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沈阳化工大学材料科学与工程学院沈阳 110142