Citation: ZHU Guoli, YIN Fangchao, WANG Li, ZHANG Min, JIANG Lei, QIN Jianhua. Microdevice for the investigation of high-glucose induced lifespan and the protective effect of polydatin in C. elegans[J]. Chinese Journal of Chromatography, ;2016, 34(2): 140-145. doi: 10.3724/SP.J.1123.2015.10009 shu

Microdevice for the investigation of high-glucose induced lifespan and the protective effect of polydatin in C. elegans

1.
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: QIN Jianhua,
Received Date: 9 October 2015

Fund Project: 国家自然科学基金项目(81201689,81273483) (81201689,81273483)中国科学院知识创新工程重要方向项目(KJCX2-YW-H18) (KJCX2-YW-H18)科技部国际合作项目(2015DFA00740). (2015DFA00740)

Caenorhabditis elegans (C. elegans) has been widely used as a model organism for biomedical research due to its sufficient homology with human at molecular or genomic level. In this work, we describe a microfluidic device not only to investigate the response of C. elegans including lifespan and oxidative stress, but also to evaluate the protective effect of polydatin induced by high-glucose condition. It was found that the mean lifespan of worms was significantly reduced and the oxidative stress protein GST-4 was increased in worms that are subjected to high glucose. However, a certain dose of polydatin could weaken the increased oxidative stress induced by high-glucose and extend the lifespan, indicating the protective effect of polydatin against the toxic of high-glucose. The established approach is simple to operate, easy for realtime imaging and multiparatemer evaluations in parallel, providing a potential platform for drug evaluation/screening in a high throughput format at single animal resolution.
- microfluidic,
- Caenorhabditis elegans,
- glucose,
- polydatin
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