Citation: SUN Jian, LI Limin, HU Qing, MAO Xiuhong, JI Shen. Determination of ginkgolic acids in the ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(2): 184-188. doi: 10.3724/SP.J.1123.2015.08027 shu

Determination of ginkgolic acids in the ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry

1.
Shanghai Institute for Food and Drug Control, Shanghai 201203, China

Corresponding author: JI Shen,
Received Date: 24 August 2015

Fund Project:

An analytical method was developed for the determination of ginkgolic acids in ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry. A chromatographic column, Agilent Poroshell 120 EC-C18(50 mm×3.0 mm, 2.7 μ m), was used with methanol-1% acetic acid (90 : 10, v/v) as the mobile phase. The ginkgo acids were detected by electrospray ionization mass spectrometry in negative mode with multiple reaction monitoring (MRM) mode. Ginkgo acids C13 : 0, C15 : 1 and C17 : 1 possessed good linear correlations in the mass concentration range from 2 to 200 μ g/L, with the correlation coefficients more than 0.999. The mean recoveries at the spiked levels of 5, 20 and 100 μ g/L were in the range of 86.3%-114.3%, and the RSDs were 0.5%-13.6%. The limits of detection and quantification were 0.003-0.08 μ g/g and 0.01-0.19 μ g/g, respectively. The method was successfully applied to the analysis of actual samples.
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沈阳化工大学材料科学与工程学院沈阳 110142