Citation: LI Shiyan, WANG Yang, ZHOU Fan, ZHENG Chongying, ZHANG Haiqi, HE Zhongyang, HE Xin. Rapid screening and confirmation of non-target pigment in Chinese softshell turtle by liquid chromatography coupled to time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(12): 1251-1256. doi: 10.3724/SP.J.1123.2015.08008 shu

Rapid screening and confirmation of non-target pigment in Chinese softshell turtle by liquid chromatography coupled to time of flight mass spectrometry

LI Shiyan ¹ ,
WANG Yang ¹ ,
ZHOU Fan ¹ ,
ZHENG Chongying ¹ ,
ZHANG Haiqi ¹ ,
HE Zhongyang ^1,, ,
HE Xin ²

1.
1. Aquatic Products Quality Inspection Center of Zhejiang Province, Hangzhou 310023, China;

Corresponding author: HE Zhongyang,
Received Date: 9 August 2015

Fund Project: 浙江省科技厅公益技术研究农业项目(2013C32062,2015C32029) (2013C32062,2015C32029)浙江省自然科学基金项目(LQ14C190001) (LQ14C190001)

A method of non-target pigment screening in Chinese softshell turtle has been established by using liquid chromatography coupled to time of flight mass spectrometry (LC-Q-TOF MS). After being purified by a simple acetonitrile extraction work, the non-target pigment in 20 Chinese softshell turtle samples was detected by liquid chromatography-photodiode array detection (LC-DAD). The S7 sample, which has a strong spectral response, was chosen to extract the mass spectrometry information of the non-target pigment on different gradient elution conditions. In order to get the characteristic molecular mass ion (564.39773 Da and 564.39561 Da) of the non-target pigment, qualitative spectral full scan with negative sample was used. The molecular formula generation data and the literature information prompted speculation that the non-target pigment was canthaxanthin with the formula of C₄₀H₅₂O₂. By comparing the canthaxanthin standard material MS/MS information, the result was confirmed accurate. A strategy of LC-Q-TOF MS method for the qualitative analysis of unknown compounds is discussed, and the results indicated that the described method can be effectively applied to qualitative analysis for non-target pigment in Chinese softshell turtle.
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