Citation: LV Yayao, HAO Feiran, WANG Huanhuan, FU Bin, QIAN Xiaohong, ZHANG Yangjun. A novel multiple-channel apparatus for packing capillary chromatographic column and its application[J]. Chinese Journal of Chromatography, ;2015, 33(11): 1155-1162. doi: 10.3724/SP.J.1123.2015.06021 shu

A novel multiple-channel apparatus for packing capillary chromatographic column and its application

1.
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China

Corresponding author: ZHANG Yangjun,
Received Date: 16 June 2015

Fund Project: 国家重大科学计划项目(2012CB910603) (2012CB910603)国家高技术研究发展规划项目(2012AA020202) (2012AA020202)国家重大科学仪器设备开发专项项目(2012YQ12004407,2011YQ030139,2011YQ06008408,2013YQ14040506) (2012YQ12004407,2011YQ030139,2011YQ06008408,2013YQ14040506)国家自然科学基金项目(21275519,20735005,31100591). (21275519,20735005,31100591)

A novel multiple-channel apparatus for packing capillary chromatographic column was designed and manufactured for packing six capillary chromatographic columns with close column efficiency at the same time. Briefly, it consists of a magnetic stirrer, a liquid chromatographic pump and a multiple-channel can. The reagents used for preparing ODS (C18) slurry and stirring condition of the magnetic stirrer were optimized in the study. Two batches of capillary chromatographic columns were packed under the optimum condition, and these packed capillary chromatographic columns were evaluated in the terms of peak capacity, sequence coverage, retention times of three peptide ions and column pressure using the tryptic digest of a bovine serum albumin (BSA) and detected by LC-MS in electrospray ionization (ESI) mode. The experimental results showed that the six capillary chromatographic columns packed at the same time had close column efficiencies, however, the column efficiencies of twelve capillary chromatographic columns packed at two times were significantly different. In addition, there was no significant column efficiency difference when packing one or six capillary chromatographic columns at the same time. The multiple-channel apparatus designed by us is simple, time-saving, and can be applied to pack capillary chromatographic columns with similar column efficiencies, thus it is of evident advantage over traditional one-channel apparatus.
- capillary liquid chromatography-mass spectrometry (CLC-MS),
- capillary chromatographic column,
- packing column apparatus,
- multiple-channel
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